A novel chimeric antibody with circular network characteristics: autobody.
A novel chimeric antibody with circular network characteristics: autobody.
复制标题
一种具有环状网络特征的新型嵌合抗体：autobody。
DOI：
10.1111/j.1749-6632.1986.tb20861.x
复制
发表时间：
1986
影响因子：
5.2
通讯作者：
Kohler,H
中科院分区：
文献类型：
--
作者：
Kang,CY;Kohler,H
According to the network theory,’we must conceive the coexistence of one paratope and one or more idiotope sites on each antibody molecule. We have recentlyzv3 analyzed the topographical distribution of idiotope determining regions (IDR) within the three-dimensional structure of the Ig molecule. From these studies, it becomes evident that idiotopic determinants are located in minidomains. The relationship of IDR domains with respect to CDR (paratope) shows independent expression of IDR specificities. Some of the IDR domains overlap with CDR structures, while other IDRs are separated from the paratope.IDRs express idiotypic determinants that are recognized by anti-idiotypic antibodies. However, IDRs also can mimic structures of nominal antigen. These antigenmimicking IDRs are called the internal images of antigen. The topographical separation and the functional independence of paratope and IDRs permit the coexpression of a given paratope and its complementary internal image structure, the internal antigen. For example, Bona and colleagues4 described a peculiar anti-idiotypic antibody that reacts not only with the corresponding idiotope, but also with the epitope of the original antigen. They reported this antibody as “epibody,” so as to indicate the presence of an idiotypic determinant that mimics the epitope structure of the antigen for the idiotype (Abl). From these considerations, it should be possible that one antibody molecule carries the internal antigen image and at the same time has a binding site for the antigen, internal or nominal. The coexpression of paratope and internal antigen image on the same Ig molecule has important implications for the network theory, as well as for the rational design of so-called idiotope vaccines. 335 While working on idiotype vaccines, we discovered a monoclonal anti-idiotypic antibody that exhibits the so-called epibody characteristics. More importantly, we found that this particular anti-idiotypic antibody forms complexes with itself. This self-binding is completely inhibited by the original epitope. Because this antibody is syngeneic in origin, one must envision the possibility that such epibodies can occur
