Nature and anisotropy of cortical forces orienting Drosophila tissue morphogenesis
Nature and anisotropy of cortical forces orienting Drosophila tissue morphogenesis
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DOI：
10.1038/ncb1798
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发表时间：
2008-12-01
影响因子：
21.3
通讯作者：
Lenne, Pierre-Francois
中科院分区：
文献类型：
--
作者：
Rauzi, Matteo;Verant, Pascale;Lenne, Pierre-Francois
发育中的胚胎和器官的形态发生依赖于细胞重塑与邻近细胞接触的能力。使用定量建模和激光纳米解剖，我们探讨了形态发生过程的机制，即果蝇胚胎的伸长，这是由极化细胞邻居交换引起的。我们表明，顶端细胞连接处的皮质张力的各向异性足以驱动组织伸长。我们通过使用各种组织描述符比较计算机数据和体内数据来估计其价值。肌动球蛋白网络的纳米解剖表明张力是各向异性分布的并且取决于肌球蛋白 II 的积累。纳米解剖后的连接松弛也表明皮质弹力在此过程中占主导地位。有趣的是，顶点位置（三个或更多单元相交的点）的波动促进了邻居交换。我们描述了亚细胞拉伸活性极化连接重塑的贡献，以及组织伸长过程中顶点波动的许可作用。
The morphogenesis of developing embryos and organs relies on the ability of cells to remodel their contacts with neighbouring cells. Using quantitative modelling and laser nano-dissection, we probed the mechanics of a morphogenetic process, the elongation of Drosophila melanogaster embryos, which results from polarized cell neighbour exchanges. We show that anisotropy of cortical tension at apical cell junctions is sufficient to drive tissue elongation. We estimated its value through comparisons between in silico and in vivo data using various tissue descriptors. Nano-dissection of the actomyosin network indicates that tension is anisotropically distributed and depends on myosin II accumulation. Junction relaxation after nano-dissection also suggests that cortical elastic forces are dominant in this process. Interestingly, fluctuations in vertex position ( points where three or more cells meet) facilitate neighbour exchanges. We delineate the contribution of subcellular tensile activity polarizing junction remodelling, and the permissive role of vertex fluctuations during tissue elongation.