IMPAIRMENT OF LONG-TERM POTENTIATION IN RAT HIPPOCAMPUS FOLLOWING CHRONIC ETHANOL TREATMENT
IMPAIRMENT OF LONG-TERM POTENTIATION IN RAT HIPPOCAMPUS FOLLOWING CHRONIC ETHANOL TREATMENT
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DOI：
10.1016/0006-8993(84)91072-2
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发表时间：
1984-01-01
期刊：
影响因子：
2.9
通讯作者：
CARLEN, PL
中科院分区：
文献类型：
--
作者：
DURAND, D;CARLEN, PL
采用离体海马片制备方法，研究了慢性乙醇处理对大鼠长时程增强（LTP）的影响。给大鼠随意提供含乙醇或对照液体饮食。一组动物在测试前总共吃了9个月的日粮，而另一组动物在测试前吃了7个月的日粮，并允许无乙醇期2个月。以25、50、100和200 Hz的4组脉冲，间隔10 min，对辐射层进行正交刺激，诱导CA1区LTP。在停药前和停药后2个月，乙醇喂养动物中增强> 15%的切片数量明显减少。在停药前对乙醇饲养的动物进行测试时，种群峰值幅度的平均百分比增幅显著降低，但在停药2个月后没有发现显著差异，表明有一定的恢复。讨论了慢性乙醇诱导LTP抑制的可能机制。
The effect of chronic ethanol treatment on long-term potentiation (LTP), a possible substrate for memory, was studied in rats using the in vitro hippocampal slice preparation. Rats were provided ad libitum access to an ethanol-containing or control liquid diet. One group of animals received the diet for a total period of 9 mo. before testing, while a second group received the diet for 7 mo. and was allowed a 2 mo. ethanol-free period before testing. LTP was induced in the CA1 region by orthodromic stimulation of the stratum radiatum with 4 stimulus trains of 200 pulses each at 25, 50, 100 and 200 Hz separated by 10 min intervals. The number of slices with potentiation > 15% was significantly smaller in the ethanol-fed animals both before and following the 2 mo. withdrawal period. The average percent increase in the amplitude of the population spike was significantly decreased in the ethanol-fed animals when tested before withdrawal but no significant difference was detected following the 2 mo. withdrawal period, suggesting some recovery. The possible mechanisms mediating the chronic ethanol-induced depression of LTP are discussed.