Glial reactivity and impaired glutamate metabolism in short-term experimental diabetic retinopathy
Glial reactivity and impaired glutamate metabolism in short-term experimental diabetic retinopathy
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DOI：
10.2337/diabetes.47.5.815
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发表时间：
1998-05-01
期刊：
影响因子：
7.7
通讯作者：
Strother, JM
中科院分区：
文献类型：
--
作者：
Lieth, E;Barber, AJ;Strother, JM
糖尿病视网膜病变的早期病理生理学和神经和血管功能障碍的参与尚不清楚。神经胶质细胞为视网膜神经元和血管提供结构和代谢支持，细胞在某些损伤状态下产生反应性，因此我们使用链脲佐菌素短期糖尿病视网膜病变大鼠模型研究糖尿病发病后最初几个月视网膜的神经胶质反应性和其他神经胶质功能。通过双位点酶联免疫吸附试验，我们检测了中间丝胶质纤维酸性蛋白（GFAP）的表达。1个月后，GFAP基本不变，但在糖尿病开始3个月内，GFAP明显诱导，增加了5倍（P < 0.04）。免疫组化染色显示GFAP在星形胶质细胞和Muller细胞中均有诱导作用。与神经胶质细胞功能障碍一致，用同位素色谱法测定，糖尿病大鼠视网膜将谷氨酸转化为谷氨酰胺的能力降低到对照组的65% (P
The early pathophysiology of diabetic retinopathy and the involvement of neural and vascular malfunction are poorly understood. Glial cells provide structural and metabolic support for retinal neurons and blood vessels, and the cells become reactive in certain injury states, We therefore used the streptozotocin rat model of short-term diabetic retinopathy to study glial reactivity and other glial functions in the retina in the first months after onset of diabetes. With a two-site enzyme-linked immunosorbent assay, we measured the expression of the intermediate filament glial fibrillary acidic protein (GFAP). After 1 month, GFAP was largely unchanged, but within 3 months of the beginning of diabetes, it was markedly induced, by fivefold (P < 0.04). Immunohistochemical staining showed that the GFAP induction occurred both in astrocytes and in Muller cells. Consistent with a glial cell malfunction, the ability of retinas to convert glutamate into glutamine, assayed chromatographically with an isotopic method, was reduced in diabetic rats to 65% of controls (P