Enabled primarily controls filopodial morphology, not actin organization, in the TSM1 growth cone in Drosophila.
Enabled primarily controls filopodial morphology, not actin organization, in the TSM1 growth cone in Drosophila.
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DOI：
10.1091/mbc.e23-01-0003
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发表时间：
2023-07-01
影响因子：
3.3
通讯作者：
Giniger, Edward
中科院分区：
文献类型：
--
作者：
Fang, Hsiao Yu;Forghani, Rameen;Clarke, Akanni;McQueen, Philip G.;Chandrasekaran, Aravind;O'Neill, Kate M.;Losert, Wolfgang;Papoian, Garegin A.;Giniger, Edward
Ena/VASP蛋白是一种进行性肌动蛋白聚合酶，在动物的胚胎发育过程中，许多形态发生过程（包括轴突生长和引导）都需要这种聚合酶。在这里，我们使用在体内的形态和肌动蛋白分布的实时成像，以确定Ena在促进果蝇翅膀的TSM 1轴突的生长中的作用。更改Ena活动会导致TSM 1的停滞和路由错误。我们的数据表明，Ena在这个生长锥中对丝状伪足形态有重大影响，但对肌动蛋白分布只有适度的影响。这与Ena的主要调节剂Abl酪氨酸激酶形成对比，Abl酪氨酸激酶先前显示出对肌动蛋白具有深远的影响，而对TSM 1生长锥形态仅具有温和的影响。我们解释这些数据表明，Ena在这个轴突的主要作用可能是连接肌动蛋白的质膜的形态发生过程，而不是调节肌动蛋白组织本身。这些数据还表明，一个关键的作用，Ena，作用下游的Abl，可能是保持一致的组织和可靠的进化的生长锥结构，即使Abl的活动变化，在环境中的指导线索。
Ena/VASP proteins are processive actin polymerases that are required throughout animal phylogeny for many morphogenetic processes, including axon growth and guidance. Here we use in vivo live imaging of morphology and actin distribution to determine the role of Ena in promoting the growth of the TSM1 axon of the Drosophila wing. Altering Ena activity causes stalling and misrouting of TSM1. Our data show that Ena has a substantial impact on filopodial morphology in this growth cone but exerts only modest effects on actin distribution. This is in contrast to the main regulator of Ena, Abl tyrosine kinase, which was shown previously to have profound effects on actin and only mild effects on TSM1 growth cone morphology. We interpret these data as suggesting that the primary role of Ena in this axon may be to link actin to the morphogenetic processes of the plasma membrane, rather than to regulate actin organization itself. These data also suggest that a key role of Ena, acting downstream of Abl, may be to maintain consistent organization and reliable evolution of growth cone structure, even as Abl activity varies in response to guidance cues in the environment.
影响因子：
3.7
作者：
Gonçalves-Pimentel C;Gombos R;Mihály J;Sánchez-Soriano N;Prokop A
通讯作者：
Prokop A
影响因子：
2.7
作者：
Fleming, Tinya;Chien, Shih-Chieh;Vanderzalm, Pamela J.;Dell, Megan;Gavin, Megan K.;Forrester, Wayne C.;Garriga, Gian
通讯作者：
Garriga, Gian
影响因子：
3.6
作者：
Norris AD;Dyer JO;Lundquist EA
通讯作者：
Lundquist EA