Human sperm endothelial nitric oxide synthase expression: correlation with sperm motility
Human sperm endothelial nitric oxide synthase expression: correlation with sperm motility
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DOI：
10.1016/s0015-0282(98)00382-3
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发表时间：
1998-12-01
影响因子：
6.7
通讯作者：
Schlegel, PN
中科院分区：
文献类型：
--
作者：
O'Bryan, MK;Zini, A;Schlegel, PN
目的：研究内皮型一氧化氮合酶(ENOS)在人精子上的表达特征，探讨精子eNOS表达与精子功能的关系。设计：前瞻性、观察性研究。地点：大学不育临床。患者(S)：12例非无精子症不育患者(S)：对12例非无精子症不育患者精液标本进行不连续Percoll梯度分离。在Percoll梯度分离的精子中，精子上的内皮型一氧化氮合酶染色与精子活力相关。内皮型一氧化氮合酶蛋白用先前鉴定的单抗进行检测。将对照切片与预先吸附的抗体或小鼠免疫球蛋白G孵育。主要结果指标(S)：eNOS在人精子上的定位及其与精子运动的关系。结果(S)：形态正常的精子顶体后和赤道eNOS免疫组化染色。然而，形状异常的精子通常在中段和/或头部区域表现出异常染色。ENOS免疫染色异常的精子百分率与活动精子百分率(r/bar=-.46)呈显著负相关。结论：eNOS在人精子中的特异性定位提示一氧化氮可能参与了精子的正常生理。然而，精子eNOS表达的异常模式与精子活力降低有关，可能是通过产生过量的细胞毒性氧化剂。(Fertil Steril(R)1998；70：1143-7.(C)1998年美国生殖医学学会。)。
Objective: To characterize the pattern of endothelial nitric oxide synthase (eNOS) expression on human spermatozoa and to determine whether sperm eNOS expression correlates with sperm function.Design: Prospective, observational study.Setting: University infertility clinic.Patient(s): Twelve nonazoospermic infertile men.Intervention(s): Semen samples (n = 12) obtained from nonazoospermic infertile men were fractionated on discontinuous Percoll gradients. Endothelial nitric oxide synthase staining on spermatozoa was correlated with sperm motility in Percoll gradient-fractionated spermatozoa. Endothelial nitric oxide synthase protein was detected with the use of a previously characterized monoclonal antibody. Control slides were incubated with preabsorbed antibody or mouse immunoglobulin G.Main Outcome Measure(s): Localization of eNOS on human spermatozoa and correlation between the pattern of sperm eNOS expression and sperm motility.Result(s): Morphologically normal spermatozoa exhibited postacrosomal and equatorial eNOS immunostaining. However, abnormally shaped spermatozoa often exhibited aberrant staining tin the midpiece and/or head region). A significant negative correlation was observed between the percentage of sperm with aberrant eNOS immunostaining and the percentage of motile sperm ((r) over bar = -.46).Conclusion(s): The specific localization of eNOS to human spermatozoa suggests that nitric oxide may be involved in normal sperm physiology. However, aberrant patterns of sperm eNOS expression are associated with decreased sperm motility, possibly through the generation of excessive cytotoxic oxidants. (Fertil Steril(R) 1998;70:1143-7. (C) 1998 by American Society for Reproductive Medicine.).