Transcription factor AP-2 is an essential and direct regulator of epidermal development in Xenopus
Transcription factor AP-2 is an essential and direct regulator of epidermal development in Xenopus
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DOI：
10.1006/dbio.2002.0621
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发表时间：
2002-05-01
影响因子：
2.7
通讯作者：
Sargent, TD
中科院分区：
文献类型：
--
作者：
Luo, T;Matsuo-Takasaki, M;Sargent, TD
哺乳动物转录因子AP-2 α（XAP-2）的非洲爪蟾同源物的表达在中胚转变后不久在整个动物半球被激活，并在BMP信号调节的控制下，在原肠胚形成结束时局限于预期的表皮。在未来的神经嵴区的表达升高开始在这个时候。XAP-2的异位表达可以恢复神经化外胚层中表皮基因的转录，无论是在外胚层外植体还是在完整的胚胎中。同样，通过注射反义寡核苷酸或通过过表达反形态XAP-2衍生物实现的XAP-2功能的丧失导致表皮的丧失和神经基因表达的获得。这些处理也导致原肠胚形成失败。因此，AP-2是青蛙胚胎中正常表皮发育和形态发生所需的外胚层决定的关键调节剂。(C)2002 Elsevier Science（美国）。
Expression of the Xenopus homolog of the mammalian transcription factor AP-2alpha (XAP-2) is activated throughout the animal hemisphere shortly after the midblastula transition, and becomes restricted to prospective epidermis by the end of gastrulation, under the control of BMP signal modulation. Elevated expression in the future neural crest region begins at this time. Ectopic expression of XAP-2 can restore transcription of epidermal genes in neuralized ectoderm, both in ectodermal explants and in the intact embryo. Likewise, loss of XAP-2 function, accomplished by injection of antisense oligonucleotides or by overexpression of antimorphic XAP-2 derivatives, leads to loss of epidermal and gain of neural gene expression. These treatments also result in gastrulation failure. Thus, AP-2 is a critical regulator of ectodermal determination that is required for normal epidermal development and morphogenesis in the frog embryo. (C) 2002 Elsevier Science (USA).