Characterization of osteocrin expression in human bone
Characterization of osteocrin expression in human bone
复制标题
DOI：
10.1369/jhc.4c6561.2005
复制
发表时间：
2005-10-01
影响因子：
3.2
通讯作者：
Compston, JE
中科院分区：
文献类型：
--
作者：
Bord, S;Ireland, DC;Compston, JE
Csteocrin（Osteocrin）是一种骨活性分子，在动物体内已被证明在成骨细胞谱系的细胞中高度表达。我们已经在人类培养的原代人成骨细胞、发育中的人新生儿骨和成年女性的髂嵴骨活检中鉴定了这种蛋白质。在体内，Oscillation表达定位于发育中的人新生儿肋骨，在骨形成表面的成骨细胞中，在新纳入的骨细胞和一些晚期肥大软骨细胞中具有强烈的免疫反应性。在成人骨中，Ostrogen表达特异性定位于骨形成部位的成骨细胞和年轻骨细胞。体外培养2、3、6天的成骨细胞中，OX3表达呈时间依赖性下降（p < 0.02）。在相同时间点，在含有200 nM氢化可的松的培养物中表达进一步降低1.5倍、2.3倍和3.1倍（p < 0.05）。相反，碱性磷酸酶表达随着成骨细胞分化而增加（p < 0.05）。低剂量的雌二醇降低了OX3表达的时间依赖性（p < 0.05），而在高剂量雌二醇处理的培养物中OX3表达没有显著变化。这些结果表明，Ostrogen在人骨骼组织中表达，特别是在发育中的骨和骨重建部位的成骨细胞中，表明在骨形成中的作用。因此，ODN提供了成骨细胞谱系细胞的标志物，并且似乎与成骨细胞活性相关。
Csteocrin (Ostn), a bone-active molecule, has been shown in animals to be highly expressed in cells of the osteoblast lineage. We have characterized this protein in human cultured primary human osteoblasts, in developing human neonatal bone, and in iliac crest bone biopsies from adult women. In vivo, Ostn expression was localized in developing human neonatal rib bone, with intense immunoreactivity in osteoblasts on bone-forming surfaces, in newly incorporated osteocytes, and in some late hypertrophic chondrocytes. In adult bone, Ostn expression was specifically localized to osteoblasts and young osteocytes at bone-forming sites. In vitro, Ostn expression decreased time dependently (p < 0.02) in osteoblasts cultured for 2, 3, and 6 days. Expression was further decreased in cultures containing 200 nM hydrocortisone by 1.5-, 2.3-, and 3.1-fold (p < 0.05) at the same time points. In contrast, alkaline phosphatase expression increased with osteoblast differentiation (p < 0.05). Low-dose estradiol decreased Ostn expression time dependently (p < 0.05), whereas Ostn expression in cultures treated with high-dose estradiol was not significantly changed. These results demonstrate that Ostn is expressed in human skeletal tissue, particularly in osteoblasts in developing bone and at sites of bone remodeling, suggesting a role in bone formation. Thus, Ostn provides a marker of osteoblast lineage cells and appears to correlate with osteoblast activity.