Hair cell recovery in mitotically blocked cultures of the bullfrog saccule
Hair cell recovery in mitotically blocked cultures of the bullfrog saccule
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DOI：
10.1073/pnas.97.22.11722
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发表时间：
2000-10-24
影响因子：
11.1
通讯作者：
Naeger, RA
中科院分区：
文献类型：
--
作者：
Baird, RA;Burton, MD;Naeger, RA
许多非哺乳类脊椎动物的毛细胞是通过支持细胞祖细胞的有丝分裂和产生的后代分化成新的毛细胞和支持细胞而再生的。最近的研究表明，氨基糖苷类药物引起的前庭器官损伤后，毛细胞的非有丝分裂恢复也可以发生。使用毛细胞和支持细胞免疫细胞化学标记物，我们使用共聚焦和电子显微镜来检查牛蛙球囊有丝分裂阻滞培养物中庆大霉素处理后受损毛细胞的命运和未成熟毛细胞的起源。挤压和破碎的毛细胞，经历凋亡细胞死亡，被疤痕形成所取代。在失去它们的束后，亚致死损伤的毛细胞在感觉上皮中停留很长时间，获得支持细胞样形态和免疫反应性。这些损伤模式似乎是相互排斥的，这意味着亚致死损伤的毛细胞修复它们的束。移行细胞;共表达毛细胞和支持细胞标记物的细胞，可见于由邻近支持细胞的扩张产生的瘢痕形成附近。这些细胞中的大多数具有与亚致死损伤的毛细胞相似的形态和免疫反应性。超微结构分析还显示，大多数未成熟的毛细胞有：自噬空泡，这意味着它们起源于受损的毛细胞，而不是支持细胞。一些移行细胞是参与瘢痕形成的支持细胞。在毛细胞恢复期间，支持细胞的数量也减少，这支持了以下结论：一些支持细胞经历表型转化为毛细胞，而没有居间的有丝分裂事件。
Hair cells in:many nonmammalian vertebrates are regenerated by the mitotic division of supporting cell progenitors and the differentiation of the resulting progeny into new hair cells and supporting cells. Recent studies have shown that nonmitotic hair cell recovery after aminoglycoside-induced damage can also occur in the vestibular organs. Using hair cell and supporting cell immunocytochemical markers, we have used confocal and electron microscopy to examine the fate of damaged hair cells and the origin of immature hair cells after gentamicin treatment in mitotically blocked cultures of the bullfrog saccule. Extruding and fragmenting hair cells, which undergo apoptotic cell death, are replaced by scar formations. After losing their bundles, sublethally damaged hair cells remain in the sensory epithelium for prolonged periods, acquiring supporting cell-like morphology and immunoreactivity. These modes of damage appear to be mutually exclusive, implying that sublethally damaged hair cells repair their bundles. Transitional cells; coexpressing hair cell and supporting cell markers, are seen near scar formations created by the expansion of neighboring supporting:cells. Most of these cells have morphology and immunoreactivity similar to that of sublethally damaged hair cells. Ultrastructural analysis also reveals that most immature hair cells had: autophagic vacuoles, implying that they originated from damaged hair cells rather than supporting cells. Some transitional cells are supporting cells participating in scar formations. Supporting cells also decrease in number during hair cell recovery, supporting the conclusion that some supporting cells undergo phenotypic conversion into hair cells without an intervening mitotic event.