Bone marrow adipocytes support dexamethasone-induced osteoclast differentiation
Bone marrow adipocytes support dexamethasone-induced osteoclast differentiation
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DOI：
10.1016/j.bbrc.2009.03.111
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发表时间：
2009-05-15
影响因子：
3.1
通讯作者：
Shindo, Hiroyuki
中科院分区：
文献类型：
--
作者：
Hozumi, Akira;Osaki, Makoto;Shindo, Hiroyuki
The purpose of this study was to examine the ability of bone marrow adipocytes to support osteoclast differentiation in vitro. The primary bone marrow adipocytes Were obtained front bone marrow fluid during prosthesis insertion, NF kappa-B ligand (RANKL), Osteoprotegerin (OPG), and macrophage Colony stimulating factor (M-CSF) expressions in bone marrow adipocytes with or Without dexamethasone were examined. In a co-culture system with bone marrow adipocytes and osteoclast precursor cells, osteoclast differentiation was assessed by the expression of titrate-resistant acid phosphatase (TRAP) staining. RANKL, OPG, and M-CSF mRNA expressions were confirmed in all individuals, Dexamethasone significantly induced RANKL and OPG expression. The RANKL/OPG ratio was increased by dexamethasone and was significant at 10(-7) M dexamethasone. With 10(-7) M dexamethasone, osteoclast precursor cells differentiated into multinucleated TRAP-positive cells when co-cultured with bone marrow adipocytes. The present study demonstrates for the first time that bone marrow adipocytes Call Support osteoclast differentiation in vitro. (C) 2009 Elsevier Inc. All rights reserved.
