HPV16/18 E5, a promising candidate for cervical cancer vaccines, affects SCPs, cell proliferation and cell cycle, and forms a potential network with E6 and E7
HPV16/18 E5, a promising candidate for cervical cancer vaccines, affects SCPs, cell proliferation and cell cycle, and forms a potential network with E6 and E7
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HPV16/18 E5 是宫颈癌疫苗的有希望的候选者，影响 SCP、细胞增殖和细胞周期，并与 E6 和 E7 形成潜在网络
DOI：
10.3892/ijmm.2012.1168
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发表时间：
2013-01-01
影响因子：
5.4
通讯作者：
Ma, Ding
中科院分区：
文献类型：
--
作者：
Liao, Shujie;Deng, Dongrui;Ma, Ding
纺锤体检查点蛋白（spindle checkpoint proteins，SCP）在细胞有丝分裂和减数分裂过程中检测到姐妹染色单体的存在，对细胞的增殖和分化至关重要。因此，SCP在肿瘤发生中的作用越来越受到重视。增加注意力。在这项研究中，我们分析了Bub 1和Mad 2在宫颈癌（CC）的发展过程中的临床样本的免疫组织化学（IHC）的表达，我们探讨了Bub 1/Mad 2与不同蛋白质的相互作用，通过免疫沉淀（IP）。此外，我们分析了人乳头瘤病毒（HPV）16/18 E5的四种不同细胞模型的特征。我们证明，随着CC的进展，在HPVE 5的影响下，Bub 1和Mad 2的表达逐渐减少。HPV 16/18 E5的过表达显著增加细胞增殖，以及S期细胞的百分比。此外，在E5表达细胞中，p21、Bub 1和Mad 2的水平显著降低。因此，HPV 16/18 E5在癌发生中起关键作用，并且是CC治疗的潜在治疗靶点。
The spindle checkpoint proteins (SCPs), which sense the existence of misaligned sister chromatids during mitosis and meiosis, are essential for cell proliferation and differentiation. Therefore, the role of SCPs in carcinogenesis is gaining. increased attention. In this study, we analysed the expression of Bub1 and Mad2 in clinical samples by immunohistochemistry (IHC) during the development of cervical cancer (CC), and we explored the interaction of Bub1/Mad2 with different proteins through immunoprecipitation (IP). Furthermore, we analysed the characteristics of four different cell models of human papillomavirus (HPV)16/18 E5. We demonstrated that with the progression of CC, the expression of Bub1 and Mad2 was gradually reduced under the influence of HPVE5. Overexpression of HPV16/18 E5 significantly increased cell proliferation, as well as the percentage of cells in the S phase. In addition, the levels of p21, Bub1 and Mad2 were markedly decreased in E5-expressing cells. Therefore, HPV16/18 E5 plays a critical role in carcinogenesis and is a potential therapeutic target in CC treatment.