GENOTYPE-SPECIFIC DIFFERENCES IN CHILLING TOLERANCE OF MAIZE IN RELATION TO CHILLING-INDUCED CHANGES IN WATER STATUS AND ABSCISIC-ACID ACCUMULATION
GENOTYPE-SPECIFIC DIFFERENCES IN CHILLING TOLERANCE OF MAIZE IN RELATION TO CHILLING-INDUCED CHANGES IN WATER STATUS AND ABSCISIC-ACID ACCUMULATION
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DOI：
10.1111/j.1399-3054.1993.tb01383.x
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发表时间：
1993-08-01
影响因子：
6.4
通讯作者：
DORFFLING, K
中科院分区：
文献类型：
--
作者：
CAPELL, B;DORFFLING, K
以4个耐冷性不同的玉米自交系为材料，研究了低温前、中、后不同时期水分状况和脱落酸（阿坝）水平的变化。在24/22度-C（白天/夜晚），70%的相对湿度和12小时的光周期与200 mumol m-2 s-1的荧光灯管施肥土壤中的幼苗。在2周龄时，将植物在14/12 ℃下调理4天，然后在5/3 ℃下冷却5天。其他条件（相对湿度、量子通量、光周期）不变。冷却期后，将植物转移到原始条件下进行恢复。以第3叶为材料，研究叶片坏死、离子流出、蒸腾、水分状况和阿坝积累的变化。观察到4个品系之间的耐冷性显著差异，如通过坏死叶面积、离子流出和全株存活来估计。在所有基因型中，在5/3 ℃下，调节显著提高了对低温的耐受性。在5/3 ℃的低温下，耐冷性低的基因型比耐冷性高的基因型具有更低的水分和渗透势。这些差异与较高的蒸腾速率和较低的扩散阻力值更敏感的线。在5/3摄氏度的低温胁迫期间，阿坝水平翻了两番。在14/12 ℃条件下处理期间，仅可测量到小幅升高。然而，在随后的冷却过程中，空调增强阿坝的上升。阿坝积累在两个线具有较高的耐冷性被触发在较高的叶水势，并达到较高的水平比耐冷性较差的线。我们的结论是，在玉米耐冷性相关的能力，快速和显着形成阿坝作为一种保护剂对冷害。
Four inbred maize lines differing in chilling tolerance were used to study changes in water status and abscisic acid (ABA) levels before, during and after a chilling period. Seedlings were raised in fertilized soil at 24/22-degrees-C (day/night), 70% relative humidity, and a 12-h photoperiod with 200 mumol m-2 s-1 from fluorescent tubes. At an age of 2 weeks the plants were conditioned at 14/12-degrees-C for 4 days and then chilled for 5 days at 5/3-degrees-C. The other conditions (relative humidity, quantum flux, photoperiod) were unchanged. After the chilling period the plants were transferred to the original conditions for recovery. The third leaves were used to study changes in leaf necrosis, ion efflux, transpiration, water status and ABA accumulation. Pronounced differences in chilling tolerance between the 4 lines as estimated by necrotic leaf areas, ion efflux and whole plant survival were observed. Conditioning significantly increased tolerance against chilling at 5/3-degrees-C in all genotypes. The genotypes with low chilling tolerance had lower water and osmotic potentials than the more tolerant genotypes during a chilling period at 5/3-degrees-C. These differences were related to higher transpiration rates and lower diffusive resistance values of the more susceptible lines. During chilling stress at 5/3-degrees-C ABA levels were quadrupled. Only a small rise was measurable during conditioning at 14/12-degrees-C. However, conditioning enhanced the rise of ABA during subsequent chilling. ABA accumulation in the two lines with a higher chilling tolerance was triggered at a higher leaf water potential and reached higher levels than in the less tolerant lines. We conclude that chilling tolerance in maize is related to the ability for fast and pronounced formation of ABA as a protective agent against chilling injury.