Cryopreservation of cell-containing poly(ethylene) glycol hydrogel microarrays
Cryopreservation of cell-containing poly(ethylene) glycol hydrogel microarrays
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DOI：
10.1021/bp049551
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发表时间：
2005-05-01
影响因子：
2.9
通讯作者：
Pishko, MV
中科院分区：
文献类型：
--
作者：
Itle, LJ;Pishko, MV
在这里，我们描述了哺乳动物细胞含有水凝胶的制造和保存。微阵列在药物筛选和病原体检测中具有潜在的应用。在硅衬底上制造含有鼠成纤维细胞的水凝胶微结构，并进行“阶段下降”冷冻过程。在冷冻过程的各个阶段测定凝胶内永生和原代胚胎鼠成纤维细胞的存活率，表明包埋在水凝胶微结构中的细胞在整个过程中保持活力。当与经受相同冷冻过程的永生化贴壁培养物相比时，水凝胶结构内的细胞在保存期间的所有阶段具有更高的细胞活力。最后，使用冷冻保护剂，二甲基亚砜（DMSO）的必要性进行了调查。将水凝胶中的细胞用和不用DMSO冷冻保存。DMSO的加入改变了冻融过程后的细胞活力，增强了永生化细胞系的活力，降低了原代细胞系的活力。
Here we describe the fabrication and preservation of mammalian cell-containing hydrogel. microarrays that have potential applications in drug screening and pathogen detection. Hydrogel microstructures containing murine fibroblasts were fabricated on silicon substrates and subjected to a "stage-down" freezing process. The percent viability of both immortal and primary embroyonic murine fibroblast cells within the gels was determined at various stages in the freezing process, showing that cells entrapped in hydrogel microstructures remained viable throughout the process. When compared to immortalized adherent cultures subjected to the same freezing process, cells within hydrogel structures had higher cell viabilities at all stages during preservation. Finally, the necessity of using a cryoprotectant, dimelthyl sulfoxide (DMSO), was investigated. Cells in hydrogels were cryopreserved with and without DMSO. The addition of DMSO altered cell viability after the freeze-thaw process, enhancing viability in an immortalized cell line and decreasing viability in a primary cell line.