Free-radical scavenging actions of natural antioxidants
Free-radical scavenging actions of natural antioxidants
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DOI：
10.1016/s0271-5317(98)00129-8
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发表时间：
1998-09-01
影响因子：
4.5
通讯作者：
Puntarulo, S
中科院分区：
文献类型：
--
作者：
Boveris, AD;Puntarulo, S
测量了小麦、苜蓿和银杏叶 (EGb) 提取物对 TEARS 产生的剂量依赖性抑制作用。小麦、苜蓿和 EGb 提取物的测试抗氧化剂的半抑制浓度 (IC50) 分别为 2.7+/-0.2、1.3+/-0.1 和 0.20+/-0.02 mg/ml。脂质自由基与自旋陷阱 POBN 结合产生加合物，从而产生特征 EPR 光谱。测试的抗氧化剂对脂质自由基含量的IC50，对于小麦、苜蓿和EGb提取物分别为12.4+/-0.2、7.7+/-0.3和1.20+/-0.06 mg/ml。大鼠肝微粒体在 DMPO、NADPH 和柠檬酸铁存在下生成具有 DMPO-OH 自旋加合物特征的 EPR 光谱。未添加任何清除剂的基本系统显示出 3.5 AU/mg 蛋白质的面积。存在 1.5 mg/ml EGb、4 mg/ml 小麦或苜蓿时的面积分别为 1.7+/-0.2、3.4+/-0.3 和 3.6+/-0.2 AU/mg 蛋白质。与在不存在提取物的情况下孵育的微粒体中测量的速率相比，暴露于EGb提取物的微粒体的O-2(-)生成率降低了40%。然而，向大鼠肝微粒体补充小麦或苜蓿提取物并不影响微粒体O-2(-)的产生。铁还原率不受添加任何测试提取物的影响。这里提供的数据表明，EGb 提取物能够比苜蓿和麦麸提取物更有效地限制脂质过氧化并清除大鼠肝微粒体中的脂质自由基。此外，小麦和苜蓿提取物不能抑制 O-2(-) 和 。生物膜产生 OH，表明它们在人类健康中成功用于治疗涉及自由基和氧化损伤的疾病的潜力不如使用 EGb 提取物那么有希望。 (C) 1998 爱思唯尔科学公司。
A dose-dependent inhibitory effect of wheat, alfalfa and ginkgo biloba (EGb) extracts on TEARS production was measured. The half-inhibition concentration (IC50) of the tested antioxidants were 2.7+/-0.2, 1.3+/-0.1, and 0.20+/-0.02 mg/ml for wheat, alfalfa and EGb extracts, respectively. Lipid radicals combined with the spin trap POBN resulted in adducts that gave a characteristic EPR spectrum. The IC50 of the tested antioxidants on lipid radical content, were 12.4+/-0.2, 7.7+/-0.3, and 1.20+/-0.06 mg/ml:for wheat, alfalfa and EGb extracts, respectively. Rat liver microsomes in the presence of DMPO, NADPH and iron-citrate generate an EPR spectra with characteristics of the DMPO-OH spin adduct. The basic system, without the addition of any scavenger showed an area of 3.5 AU/mg protein. The areas in the presence of 1.5 mg/ml EGb, 4 mg/ml wheat or alfalfa, were of 1.7+/-0.2, 3.4 +/-0.3, and 3.6+/-0.2 AU/mg protein, respectively. O-2(-) generation rate by the microsomes exposed to EGb extract was decreased by 40%, as compared to the rate measured in microsomes incubated in the absence of the extract. However, the supplementation of rat liver microsomes with either wheat or alfalfa extracts did not affect microsomal generation of O-2(-). Iron reduction rate was not affected by the addition of any of the tested extracts. The data presented here showed that EGb extracts were able to limit lipid peroxidation and scavenge lipid radicals in rat liver microsomes more efficiently than alfalfa and wheat bran extracts. Moreover, wheat and alfalfa extracts were not able to inhibit O-2(-) and . OH generation by biological membranes, suggesting that their potentiality to be successfully used in human health in the treatment of diseases involving free radical and oxidative damage are not as promising as that for the use of EGb extracts. (C) 1998 Elsevier Science Inc.