Effect of the Calcium Antagonist Verapamil on Necrosis following Temporary Coronary Artery Occlusion in Dogs
Effect of the Calcium Antagonist Verapamil on Necrosis following Temporary Coronary Artery Occlusion in Dogs
复制标题
钙拮抗剂维拉帕米对犬暂时冠状动脉闭塞后坏死的影响
作者：
K. Reimer;J. Lowe;R. Jennings
钙代谢可能在心肌缺血性损伤的发病机制中发挥重要作用。在暂时冠状动脉闭塞的狗身上研究了肌膜钙流抑制剂维拉帕米对心肌坏死的作用。一组狗未经治疗。第二组在冠状动脉闭塞前 10 分钟开始，在 30 分钟内静脉注射 0.8 mg/kg 维拉帕米。在第三组中，维拉帕米的剂量增加，直至并发低血压或传导异常。左冠状动脉回旋支闭塞 40 分钟，然后再灌注 2-4 天，所有狗均出现心脏坏死。实验结束时，处死动物，并通过后乳头肌的透壁切片对坏死进行组织学定量。使用较低剂量的维拉帕米进行预处理可显着减少坏死（治疗组为 14%，未治疗组为 35%），且血流动力学影响最小。尽管出现低血压和不同程度的心脏传导阻滞，但较高剂量的维拉帕米在限制心脏坏死方面甚至更有效。
Calcium metabolism may play an important role in the pathogenesis of myocardial ischemic injury. The effect of the sarcolemmal calcium flux inhibitor, verapamil, on myocardial necrosis was studied in dogs subjected to temporary coronary artery occlusion. One group of dogs was untreated. A second group was given 0.8 mg/kg verapamil intravenously over a 30 min period beginning 10 min prior to coronary occlusion. In a third group, the dose of verapamil was increased until complicated by hypotension or conduction abnormalities. Cardiac necrosis was produced in all dogs by 40 min of left circumflex coronary artery occlusion followed by 2-4 days of reperfusion. At the end of the experiment, animals were sacrificed and necrosis was quantitated histologically in transmural slices through the posterior papillary muscle. Pre-treatment with the lower dose of verapamil resulted in significantly less necrosis (14% treated vs 35% untreated) with minimal hemodynamic consequences. Higher doses of verapamil were even more effective in limiting cardiac necrosis despite the development of hypotension and varying degrees of heart block.