Molecular Detection of Tick-Borne Pathogens of the Family Anaplasmataceae in Brazilian Brown Brocket Deer (Mazama gouazoubira, Fischer, 1814) and Marsh Deer (Blastocerus dichotomus, Illiger, 1815)
Molecular Detection of Tick-Borne Pathogens of the Family Anaplasmataceae in Brazilian Brown Brocket Deer (Mazama gouazoubira, Fischer, 1814) and Marsh Deer (Blastocerus dichotomus, Illiger, 1815)
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DOI：
10.1111/j.1865-1682.2011.01278.x
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发表时间：
2012-08-01
影响因子：
4.3
通讯作者：
Ribeiro, M. F. B.
中科院分区：
文献类型：
--
作者：
Silveira, J. A. G.;Rabelo, E. M. L.;Ribeiro, M. F. B.
鹿是无形体科重要的天然贮存宿主。本研究采用巢式PCR和核苷酸测序，以评估发生的无形体科物种在23个自由生活和6个圈养标本的鹿Mazama gouazoubira和Blastocerus dichotomus在米纳斯吉拉斯州，巴西。检测血样中埃立克体属和无形体属的存在。使用nPCR测定和msp 4、msp 1和16 S rRNA基因的测序。通过使用BLAST软件与可从GenBank获得的序列进行比较来确认每个序列的同一性。93.1%（27/29）的动物感染血液寄生虫，包括边缘无形体（79.3%）、查菲埃立克体（3.4%）、牛无形体（3.4%）和无形体属（3.4%）。（分配给A。platys和A.嗜吞噬细胞菌（17.2%）。共感染率为20%（6/29）。4例（13.8%）感染A. marginale和无形体属（Anaplasma sp.），感染A. marginale和E.查菲吸虫感染1例（3.4%）。marginale和A.牛本研究的结果表明，交叉保护不会发生在这些鹿。免疫交叉反应发生时，血清进行诊断测试，因为这些细菌是密切相关的分类，加强了分子诊断的重要性，其次是核苷酸测序。
Deer are important natural reservoir hosts of Anaplasmataceae. The present study used nested PCR and nucleotide sequencing to evaluate the occurrence of Anaplasmataceae species in 23 free-living and six captive specimens of the cervids Mazama gouazoubira and Blastocerus dichotomus in Minas Gerais State, Brazil. Blood samples were tested for the presence of Ehrlichia and Anaplasma spp. using nPCR assays and sequencing of the msp4, msp1 and 16S rRNA genes. The identity of each sequence was confirmed by comparison with sequences available from GenBank using BLAST software. Of the animals investigated, 93.1% (27/29) were infected with haemoparasites including Anaplasma marginale (79.3%), Ehrlichia chaffeensis (3.4%), Anaplasma bovis (3.4%) and Anaplasma spp. (assigned to A. platys and A. phagocytophilum) (17.2%). Co-infection occurred in 20% (6/29) of the deer examined. Four (13.8%) were infected with A. marginale and Anaplasma sp., one (3.4%) was infected with A. marginale and E. chaffeensis, and one (3.4%) was infected with A. marginale and A. bovis. The results of the present study suggest that cross-protection does not occur in these deer. Immunological cross-reaction occurs when sera are tested diagnostically because these bacteria are closely related taxonomically, reinforcing the importance of molecular diagnosis followed by nucleotide sequencing.