Automated system for training and assessing string-pulling behaviors in rodents.
Automated system for training and assessing string-pulling behaviors in rodents.
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用于训练和评估啮齿动物拉绳行为的自动化系统。
DOI：
10.1101/2023.07.02.547431
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Cowen,StephenL
中科院分区：
文献类型：
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作者：
Jordan,GiannaA;Vishwanath,Abhilasha;Holguin,Gabriel;Bartlett,MitchellJ;Tapia,AndrewK;Winter,GabrielM;Sexauer,MorganR;Stopera,CarolynJ;Falk,Torsten;Cowen,StephenL
几个世纪以来，拉线任务一直被用来研究协调的双手运动行为和问题解决。牵绳学得很快，在行为学上是扎根的，并已被应用于许多物种和疾病条件。通常，拉线行为的训练是通过人工塑造和下饵来实现的。此外，伸手、抓取和拉动的行为评估通常通过劳动密集型人工视频评分来执行。据我们所知，目前还没有用于自动塑造和评估拉线行为的系统。在这里，我们描述了Panda系统(拉绳和神经数据分析)，这是一种廉价的硬件和软件系统，它利用一个连接到旋转编码器、供给器、微控制器、高速摄像机和分析软件的连续弦环，用于评估和训练拉线行为并与神经记录数据同步。我们在未植入的大鼠和在运动皮质和海马区植入电极的大鼠身上演示了这一系统，并展示了熊猫系统如何用于评估爪子运动与单个单位和局部领域活动之间的关系。我们还发现，自动化塑形程序显著提高了整体性能，老鼠在15分钟的时间里有规律地拉100米。总而言之，熊猫系统将对研究运动控制、动机和运动障碍(如帕金森氏病、亨廷顿病和中风)的研究人员普遍使用。它还将支持对涉及感觉运动整合的神经机制的研究。
String-pulling tasks have been used for centuries to study coordinated bimanual motor behavior and problem solving. String pulling is rapidly learned, ethologically grounded, and has been applied to many species and disease conditions. Typically, training of string-pulling behaviors is achieved through manual shaping and baiting. Furthermore, behavioral assessment of reaching, grasping, and pulling is often performed through labor intensive manual video scoring. No system, to our knowledge, currently exists for the automated shaping and assessment of string-pulling behaviors. Here we describe the PANDA system (Pulling And Neural Data Analysis), an inexpensive hardware and software system that utilizes a continuous string loop connected to a rotary encoder, feeder, microcontroller, high-speed camera, and analysis software for assessment and training of string-pulling behaviors and synchronization with neural recording data. We demonstrate this system in unimplanted rats and rats implanted with electrodes in motor cortex and hippocampus and show how the PANDA system can be used to assess relationships between paw movements and single-unit and local-field activity. We also found that automating the shaping procedure significantly improved overall performance, with rats regularly pulling >100 meters during a 15-minute session. In conclusion, the PANDA system will be of general use to researchers investigating motor control, motivation, and motor disorders such as Parkinson’s disease, Huntington’s disease, and stroke. It will also support the investigation of neural mechanisms involved in sensorimotor integration.