Suppression of rice seedling rot caused by Burkholderia glumae in nursery soils using culturable bacterial communities from organic farming systems
Suppression of rice seedling rot caused by Burkholderia glumae in nursery soils using culturable bacterial communities from organic farming systems
复制标题
DOI：
10.1007/s42161-022-01066-6
复制
发表时间：
2022-02-11
影响因子：
2.2
通讯作者：
Takahashi, Hideki
中科院分区：
文献类型：
--
作者：
Ando, Sugihiro;Kasahara, Masami;Takahashi, Hideki
在这项研究中，我们开发了一种有效且稳定的技术，用于抑制由水稻细菌性谷枯病菌（Burkholderia glumae）引起的水稻秧苗腐烂。该技术利用从具有病害抑制活性的水稻有机耕作（OF）育苗土壤中培养的细菌群落。当通过在营养琼脂（NA，营养丰富的条件）培养基上生长从有机耕作土壤中分离出的可培养细菌群落（CBCs）被添加到高压灭菌的常规育苗土壤中时，对于从土壤样本中获得的一些但不是所有的CBCs，观察到了对秧苗腐烂的病害抑制效果。当细菌在营养匮乏的条件下（1/1000稀释的NA培养基和1/1000稀释的营养结冷胶培养基）培养时，更频繁地观察到病害抑制效果。在营养匮乏的培养基上反复传代培养时，病害抑制活性得以保持，但在营养丰富的培养基上则不能。这些结果表明，CBCs根据其土壤来源和培养条件，具有用于病害控制处理的潜力。对CBCs组成的分析表明，伯克氏菌目（Burkholderiales）的细菌可能是病害抑制活性的原因。此外，与病害抑制效果相关的是，在施用CBCs后在根际土壤中形成的细菌群落结构稳定且能抵抗水稻细菌性谷枯病菌的入侵。尽管可培养细菌仅代表有机耕作土壤细菌种群的一部分，但CBC的施用可能模拟了有机耕作土壤的病害抑制效果。
In this study, we developed an effective and stable technique for suppressing seedling rot of rice caused by Burkholderia glumae using bacterial communities cultured from nursery soils for organic farming (OF) of rice that have disease-suppressive activity. When culturable bacterial communities (CBCs) isolated from OF soils by growth on nutrient-agar (NA, nutrient-rich conditions) medium were added to autoclaved conventional nursery soil, a disease-suppressive effect on seedling rot was observed for some, but not all the CBCs obtained from soil samples. When bacteria were cultured under nutrient-poor conditions (1/1,000 diluted NA medium and 1/1,000 diluted nutrient-gellan gum medium), a disease-suppressive effect was observed more frequently. The disease-suppressive activity was maintained during repeated subculture on nutrient-poor media, but not on nutrient-rich media. These results suggest that CBCs have potential for disease control treatments, depending on their soil source and culture conditions. Analysis of the composition of CBCs suggested that bacteria in the order Burkholderiales may be responsible for the disease-suppressive activity. Correlated with the disease-suppressive effect, furthermore, the bacterial community structures that developed in rhizosphere soils after application of CBCs were stable and resistant to B. glumae invasion. Although culturable bacteria represent only a part of the bacterial populations of OF soils, it is possible that CBC application mimics the disease-suppressive effects of OF soils.