Brain edema and intracranial hypertension in rats after total hepatectomy.
Brain edema and intracranial hypertension in rats after total hepatectomy.
复制标题
DOI：
10.1016/0016-5085(95)90208-2
复制
发表时间：
1995-04
期刊：
影响因子：
29.4
通讯作者：
S. Olafsson;J. Gottstein;A. Blei
中科院分区：
文献类型：
--
作者：
S. Olafsson;J. Gottstein;A. Blei
谷氨酰胺是由星形胶质细胞中的氨生成的，可能是急性肝衰竭时脑水肿的原因。最近的研究表明，人类肝切除术后颅内压降低，这表明坏死肝脏释放的因子可能在脑肿胀中起致病作用。本研究的目的是检查是否脑水肿和颅内高压发展hepatectomized rats.MethodsRats进行门腔静脉吻合术或假手术。在24小时时，动物进行第二次假手术或全肝切除术。处死大鼠后连续监测颅内压，测定脑皮质含水量和谷氨酰胺含量。在第二个实验中，hepatectomized和devascularized（门腔静脉吻合加肝动脉结扎）大鼠被杀死，每2小时，并在颅内高压的时间。ResultsAlthough脑水肿的发展，在两组肝功能衰竭，断流导致更高的脑水含量，尽管谷氨酰胺浓度的同等增加。颅内压增加到相似的程度，在这两个群体，但所有参数增加更早在anhepatic rats.ConclusionsHepatectomized大鼠发展脑水肿和颅内高压。该模型中的时间序列支持谷氨酰胺作为有机渗透剂的作用。此外，其他因素（例如，脑容量）可能导致肝切除大鼠的颅内高压。
Background/AimsGlutamine, generated from ammonia in astrocytes, may account for brain edema in acute liver failure. Recent studies showing decreased intracranial pressure after hepatectomy in humans suggest that factors released by the necrotic liver could play a pathogenic role in brain swelling. The aim of this study was to examine whether brain edema and intracranial hypertension develop in hepatectomized rats.MethodsRats underwent a portacaval anastomosis or a sham operation. At 24 hours, animals underwent a second sham operation or a total hepatectomy. Intracranial pressure was continuously monitored, and cortical water and glutamine contents were measured after the rats were killed. In a second experiment, hepatectomized and devascularized (portacaval anastomosis plus hepatic artery ligation) rats were killed every 2 hours and at the time of intracranial hypertension.ResultsAlthough brain edema developed in both groups with liver failure, devascularization resulted in a higher brain water content in spite of an equivalent increase in glutamine concentration. Intracranial pressure increased to a similar degree in both groups, but all parameters increased earlier in anhepatic rats.ConclusionsHepatectomized rats develop brain edema and intracranial hypertension. The temporal sequence in this model supports the role of glutamine as an organic osmolyte. In addition, other factors (e.g., brain volume) may contribute to intracranial hypertension in hepatectomized rats.