Intestinal cell respiration is influenced by animal age, strain, and feeding status.
Intestinal cell respiration is influenced by animal age, strain, and feeding status.
复制标题
肠细胞呼吸受动物年龄、品系和喂养状态的影响。
DOI：
10.1093/geronj/49.1.b22
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Hudes,M
中科院分区：
文献类型：
--
作者：
Fleming,SE;Fitch,MD;Hudes,M
本研究的目的是评估衰老和禁食状态对体外空肠和结肠细胞氧化底物的影响，并确定这些因素的作用是否会受到大鼠品系的影响。采用Fischer 344 （F344）和Fischer × Brown Norway （F × BN）两种菌株的幼龄（4个月）和老龄（24个月）雄性大鼠进行48小时禁食和自由喂养。在实验的早晨，从空肠和结肠中取出细胞，将这些悬浮液的等分液放在含有微量14c标记同位素的5毫米浓度的底物中孵育。孵育60分钟后，收集14co2并定量测定底物氧化。研究了葡萄糖、谷氨酰胺和3-羟基丁酸在空肠和结肠细胞中的氧化作用，而乙酸和丁酸在结肠细胞中的氧化作用。空腹48小时的小鼠空肠细胞对葡萄糖的氧化作用低于饲喂动物，但饲喂状态对小鼠空肠或结肠细胞对其他底物的氧化作用无显著影响。当从幼龄动物身上提取空肠和结肠细胞时，F344菌株与F × BN菌株的底物氧化无差异。然而，在老龄动物中，由于大鼠品系的差异变得明显，F344老龄动物的几种底物氧化率高于F × BN老龄动物。特别是，老年F344大鼠的结肠细胞比年轻F344大鼠的细胞更容易氧化醋酸盐、丁酸盐和3-羟基丁酸盐，而年龄对F × BN菌株的差异不显著。
The objectives of this study were to evaluate the influence of aging and the fasted vsfed state on substrate oxidation by jejunal and colonic cells in vitro, and to determine whether the effects of these factors would be influenced by rat strain. Young (4 mo) and aged (24 mo) male rats of the Fischer 344 (F344) and Fischer × Brown Norway (F × BN) strains were used either following a 48-hr fast or in the ad libitum fed state. On the morning of experimentation, cells were removed from segments of the jejunum and colon and aliquots of these suspensions were incubated in 5 mM concentrations of substrates containing trace quantities of14C-labeled isotopes. Following 60 min of incubation,14CO2was collected and quantified to determine substrate oxidation. The oxidation of glucose, glutamine, and 3-hydroxybutyrate was studied in jejunal and colonic cells, and the oxidation of acetate and butyrate was studied in colonic cells only. Glucose oxidation by jejunal cells was lower when cells were taken from 48-hr fasted animals than from fed animals, but the feeding status of the animal did not significantly influence oxidation of other substrates by jejunal or colonic cells. Substrate oxidation was not different for the F344 vs F × BN strains when jejunal and colonic cells were taken from young animals. Differences due to rat strain became apparent in the aged animals, however, with oxidation of several substrates being higher for the aged F344 than for the aged F × BN animals. In particular, colonic cells from aged F344 rats oxidized acetate, butyrate, and 3-hydroxybutyrate to greater extents than cells from young F344 rats, whereas differences due to age were not significant for the F × BN strain.