iTRAQ compatibility of peptide immobilized pH gradient isoelectric focusing

iTRAQ compatibility of peptide immobilized pH gradient isoelectric focusing
复制标题

DOI:
10.1002/pmic.200600782
复制
发表时间:
2007-06-01
期刊:
影响因子:
3.4
通讯作者:
Lehtio, Janne
Lehtio, Janne
中科院分区:
生物学3区
文献类型:
--
作者:
Lengqvist, Johan;Uhlen, Kristina;Lehtio, Janne

文献摘要

被引文献

相似文献

固定化pH梯度等电聚焦(IPG-IEF)。出现了一个非常有前途的替代强阳离子交换分馏作为第一个维度在猎枪蛋白质组学。本文显示了该方法与iTRAQ同位素标记的兼容性,用于相对定量和验证来自数据库搜索的序列匹配。
Immobilized pH gradient isoelectric focusing (IPG-IEF) has. emerged as a highly promising alternative to strong-cation exchange fractionation as the first dimension in shot-gun proteomics. Herein is shown the compatibility of this method with iTRAQ isotope labeling for relative quantitation and validation of sequence matches from database searching.