iTRAQ compatibility of peptide immobilized pH gradient isoelectric focusing
iTRAQ compatibility of peptide immobilized pH gradient isoelectric focusing
复制标题
DOI:
10.1002/pmic.200600782
复制
发表时间:
2007-06-01
期刊:
影响因子:
3.4
通讯作者:
Lehtio, Janne
中科院分区:
文献类型:
--
作者:
Lengqvist, Johan;Uhlen, Kristina;Lehtio, Janne
Immobilized pH gradient isoelectric focusing (IPG-IEF) has. emerged as a highly promising alternative to strong-cation exchange fractionation as the first dimension in shot-gun proteomics. Herein is shown the compatibility of this method with iTRAQ isotope labeling for relative quantitation and validation of sequence matches from database searching.