Imaging mitochondrial redox environment and oxidative stress using a redox-sensitive fluorescent protein.
Imaging mitochondrial redox environment and oxidative stress using a redox-sensitive fluorescent protein.
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DOI：
10.1272/jnms.75.66
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发表时间：
2008-04
期刊：
影响因子：
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通讯作者：
Alexander M. Wolf;S. Asoh;I. Ohsawa;S. Ohta
中科院分区：
文献类型：
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作者：
Alexander M. Wolf;S. Asoh;I. Ohsawa;S. Ohta
氧化还原敏感性绿色荧光蛋白（roGFP）是一种荧光蛋白，其中两个半胱氨酸在桶结构中相邻放置。二硫化物的形成（氧化）增加了在短波长（410 nm）的吸收，但牺牲了在较长波长（490 nm）的吸收。荧光比率指示还原/氧化，即，在特定细胞位置的氧化还原电位。
Redox-sensitive green fluorescent protein (roGFP) is a fluorescent protein in which two cysteines are placed adjacently in the barrel structure. Disulfide formation (oxidation) increases the absorption at short wavelengths (410 nm) at the expense of absorption at longer wavelengths (490 nm). The fluorescence ratio indicates reduction/oxidation, i.e., the redox potential at specific cellular locations.