Differential localization of annexins in ram germ cells: a biochemical and immunocytochemical study.
Differential localization of annexins in ram germ cells: a biochemical and immunocytochemical study.
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公羊生殖细胞中膜联蛋白的差异定位：生化和免疫细胞化学研究。
DOI：
10.1177/39.7.1830893
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
S. Weinman
中科院分区：
文献类型：
--
作者：
J. Feinberg;D. Rainteau;M. Kaetzel;J. Dacheux;J. Dedman;S. Weinman
我们使用在蛋白质印迹上特异性结合膜联蛋白的抗体，通过免疫金电子显微镜测定这些蛋白质在公羊精子细胞和精子中的分布和丰度。膜联蛋白 I 和 II 基本上存在于精子细胞的整个顶体内。在附睾成熟期间，它们分别集中在顶体后区域或顶体赤道段。它们还存在于精子鞭毛、粗纤维和纤维鞘的表面上。这些发现表明，在公羊生殖细胞成熟过程中，膜联蛋白 I 和 II 从精子细胞顶体输出到精子的结构和功能限定部分。在公羊生殖细胞中未发现膜联蛋白 III、IV 和 V。从睾丸和精子中分离出膜联蛋白 VI。在精子细胞中，它被发现与内质网和线粒体有关，但在顶体中不存在。在精子中，它仅限于鞭毛、线粒体以及粗纤维和纤维鞘。除钙调蛋白外，功能区域中三种膜联蛋白的存在可能表明精子控制和调节已知钙依赖性事件（例如鞭毛运动、顶体反应和受精）的不同方式。
We used antibodies that specifically bind annexins on Western blots to determine the distribution and abundance of these proteins in ram spermatids and sperm by immunogold electron microscopy. Annexins I and II were found essentially within the entire acrosome of spermatids. During epididymal maturation, they concentrated in the postacrosomal region or the acrosomal equatorial segment, respectively. They were also present in sperm flagellum, on the surface of the coarse fibers and fibrous sheath. These findings show that during ram germ cell maturation, annexins I and II are exported from the spermatid acrosome towards structurally and functionally defined parts of the sperm. Annexins III, IV, and V were not found in ram germ cells. Annexin VI was isolated from testis and sperm. In spermatids, it was found to be associated with endoplasmic reticulum and the mitochondria but was absent from the acrosome. In sperm, it was confined to the flagellum, the mitochondria, and on the coarse fibers and fibrous sheath. The presence of three annexins, in addition to calmodulin, in functional areas may indicate differential ways for sperm to control and regulate events that are known to be calcium dependent, such as flagellar motility, acrosome reaction, and fertilization.
35-和 67-kDa 钙调素的表达依赖于甲状腺激素。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Rainteau,DP;Weinman,SJ;Kabaktchis,CA;Smith,VL;Kaetzel,MA;Dedman,JR;Weinman,JS
通讯作者：
Weinman,JS