Visible fluorescence chemosensor for saxitoxin
Visible fluorescence chemosensor for saxitoxin
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DOI：
10.1021/jo062506r
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发表时间：
2007-03-16
影响因子：
3.6
通讯作者：
Pharr, Jennifer S.
中科院分区：
文献类型：
--
作者：
Gawley, Robert E.;Mao, Hua;Pharr, Jennifer S.
已获得了一些贝类提取物的吸收光谱，并在所有样品中在210和260 nm处以及其中一些样品在325 nm处显示出显著的吸收。这些限制排除了在测试麻痹性贝类毒素的贝类样品中使用具有类似限制的发色团。两个冠醚化学传感器具有硼氮杂二吡啉发色团已被合成，都有最大吸收在650 nm处，所有的贝类提取物是透明的。合成传感器功能无论是18-或27元冠醚环，并已被评估为麻痹性贝类毒素石房蛤毒素的可见传感器。其中之一的结合常数在3-9 × 10（5）M-1的范围内，并在40 μ M石房蛤毒素存在下在680 nm处显示超过100%的荧光增强。
Absorption spectra of a number of shellfish extracts have been obtained and reveal prominent absorptions in all samples at 210 and 260 nm and at 325 nm in some of them. These absorptions preclude the use of chromophores with similar absorptions in testing of shellfish samples for paralytic shellfish toxins. Two crown ether chemosensors featuring a boron azadipyrrin chromophore have been synthesized; both have absorption maxima at 650 nm, where all the shellfish extracts are transparent. The synthetic sensors feature either 18- or 27-membered crown ether rings and have been evaluated as visible sensors for the paralytic shellfish toxin saxitoxin. The binding constant for one of them is in the range of 3-9 x 10(5) M-1 and exhibits a fluorescence enhancement of over 100% at 680 nm in the presence of 40 mu M saxitoxin.