Mating in Heliothis virescens: transfer of juvenile hormone during copulation by male to female and stimulation of biosynthesis of endogenous juvenile hormone.
Mating in Heliothis virescens: transfer of juvenile hormone during copulation by male to female and stimulation of biosynthesis of endogenous juvenile hormone.
复制标题
DOI：
10.1002/(sici)1520-6327(1998)38:2
复制
发表时间：
1998
影响因子：
2.2
通讯作者：
Yong Ihl Park;Shengqiang Shu;S. Ramaswamy;A. Srinivasan
中科院分区：
文献类型：
--
作者：
Yong Ihl Park;Shengqiang Shu;S. Ramaswamy;A. Srinivasan
进行研究以确定烟芽夜蛾的成年雄性在交配期间是否将保幼激素（JH）转移给雌性，并且使用体外放射化学测定来确定交配是否引起异源效应，即，咽侧体（CA）刺激JH生物合成。离体条件下，3日龄交配雌虫的CA合成和释放的JH总量约为未交配雌虫CA的2.5倍。同源物中，JH II在交配雌性中表现出显著增加; JH I也增加但不显著。JH III仍然类似于处女女性。这是第一次证明蛾类交配的异向性效应。与雌性相反，CA的处女男性不产生任何JH，但辅助性腺（ASG）在3日龄的男性合成少量的JH。成虫羽化后，雄性ASG中含有约1.5 ng JH I和II，羽化后12 h增加，羽化后54 h仍保持在这个高水平。在ASG中几乎未检测到JH III。解偶联后交配雄性ASG中的JH几乎完全耗尽，24 h后恢复到与54 h龄处女雄性相当的水平。处女雌性交配囊不含任何JH，但交配的雌性囊，解偶后立即，有JH的水平与处女雄性ASG中观察到的水平相当。解偶联后6小时，交配后的雌性腔上囊中JH水平急剧下降。这些数据表明，JH转移到女性的男性。
Studies were undertaken to determine whether adult males of Heliothis virescens transfer juvenile hormone (JH) to females during copulation, and an in vitro radiochemical assay was used to determine whether mating causes an allatotropic effect, i.e., stimulation of JH biosynthesis by corpora allata (CA). In vitro, CA from 3-day-old mated females synthesized and released approximately 2.5 times total JH as that of CA from comparably aged virgin females. Of the homologues, JH II exhibited significant increase in mated females; JH I also increased but not significantly. JH III remained similar to that of virgin females. This is the first demonstration of an allatotropic effect of mating in moths. In contrast to the female, CA of virgin males did not produce any JH, but accessory sex glands (ASG) in 3-day-old males synthesized small amounts of JH. Immediately after adult emergence, male ASG contained approximately 1.5 ng JH I and II, which increased by 12 h after emergence and remained at this high level up to 54 h after emergence. JH III was barely detected in ASG. JH in ASG of mated male immediately after uncoupling was depleted almost completely, and 24 h later recovered to levels comparable to that of 54-h-old virgin male. Virgin female bursa copulatrix did not contain any JH, but mated female bursa, immediately after uncoupling, had JH at levels comparable to that observed in virgin male ASG. By 6 h after uncoupling, JH levels decreased dramatically in mated female bursa. These data suggest the transfer of JH to females by the male.