The effects of preservation on fish tissue stable isotope signatures
The effects of preservation on fish tissue stable isotope signatures
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DOI：
10.1111/j.1095-8649.2006.01226.x
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发表时间：
2006-12-01
影响因子：
2
通讯作者：
Power, M.
中科院分区：
文献类型：
--
作者：
Kelly, B.;Dempson, J. B.;Power, M.
研究了福尔马林和乙醇保存对北极红点鲑肌肉组织 δ C-13 和 δ N-15 同位素特征的影响。研究的组织样本的脂质含量范围为 3.6% 至 6.1%，与保存样本中观察到的同位素变化幅度无关。与对照样品相比，乙醇和福尔马林分别显着减少和富集保存组织的 δ C-13 同位素特征。与对照相比，乙醇并没有显着丰富 Delta N-15 特征，而福尔马林却可以。对多种物种影响的荟萃分析进一步证明了对鱼类组织的显着保存效果。对通过文献、自举或荟萃分析得出的校正因子校正保存的组织同位素特征获得的数据进行统计分析表明，校正样本同位素特征与对照样本同位素特征之间存在显着差异，或者当数据集相互回归时无法产生统一斜率。物种特异性的自举线性校正模型不会导致此类错误。结果表明，由于已知鱼类组织脂质含量和成分差异很大，因此应对鱼类使用特定物种的校正方法。因此，在解释食物网历史分析中的时间模式时，需要利用试点研究来制定修正因子，以适当调整保存效果。 (c) 2006 年作者杂志汇编 (c) 2006 年不列颠群岛渔业协会。
The effects of formalin and ethanol preservation on the delta C-13 and delta N-15 isotope signatures of Arctic charr Salvelinus alpinus muscle tissue were examined. The lipid content of the tissue samples studied ranged from 3.6 to 6.1% and was not correlated with the magnitude of observed isotopic shifts in preserved samples. Ethanol and formalin significantly depleted and enriched, respectively, the delta C-13 isotope signatures of preserved tissues when compared to control samples. Ethanol did not significantly enrich delta N-15 signatures in comparison to controls, whereas formalin did. A meta-analysis of multiple species effects further demonstrated significant preservation effects in fish tissue. Statistical analysis of data obtained by correcting preserved tissue isotope signatures with literature, bootstrapped or meta-analysis derived correction factors demonstrated significant differences between corrected and control sample isotope signatures or failure to produce a unity slope when the data sets were regressed against one another. Species-specific, bootstrapped linear correction models resulted in no such errors. Results suggest that species-specific correction methods should be used for fishes because of the known wide variation in fish tissue lipid content and composition. Accordingly, the use of pilot studies will be required to develop correction factors that properly adjust for preservation effects when interpreting temporal patterns in historic analyses of food webs. (c) 2006 The Authors Journal compilation (c) 2006 The Fisheries Society of the British Isles.