Drosophila homologs of transcriptional mediator complex subunits are required for adult cell and segment identity specification
Drosophila homologs of transcriptional mediator complex subunits are required for adult cell and segment identity specification
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DOI：
10.1101/gad.17900
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发表时间：
2000-11-15
影响因子：
10.5
通讯作者：
Bourbon, HM
中科院分区：
文献类型：
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作者：
Boube, M;Faucher, C;Bourbon, HM
基因表达特异性的起源是理解发育控制的核心问题。中介蛋白复合物调节转录起始，作为连接特定转录因子和核心RNA聚合酶II的模块接头。在这里，我们鉴定了23个人类中介基因的果蝇同源性和两个dTRAP240和dTRAP80的突变（酵母SRB4的假定的苍蝇同源性）。克隆分析表明dTRAP80在细胞生存中起着重要的作用。dTRAP240基因也是必不可少的，但缺乏其功能的细胞可以存活并正常增殖。克隆揭示了局部发育活动，包括性梳细胞身份功能。这与成像椎间盘中普遍存在的dTRAP240蛋白核积累形成对比。协同遗传相互作用支持dTRAP240和dTRAP80共享发育细胞和片段识别功能，可能在一个共同的复合体内。此外，他们确定了相同细胞/组织身份所需的同源性梳子减少产物，作为这些中介蛋白的功能伙伴。
The origins of specificity in gene expression are a central concern in understanding developmental control. Mediator protein complexes regulate transcriptional initiation, acting as modular adaptors linking specific transcription factors to core RNA polymerase II. Here, we identified the Drosophila homologs of 23 human mediator genes and mutations of two, dTRAP240 and of dTRAP80 (the putative fly homolog of yeast SRB4). Clonal analysis indicates a general role for dTRAP80 necessary for cell viability. The dTRAP240 gene is also essential, but cells lacking its function are viable and proliferate normally. Clones reveal localized developmental activities including a sex comb cell identity function. This contrasts with the ubiquitous nuclear accumulation of dTRAP240 protein in imaginal discs. Synergistic genetic interactions support shared developmental cell and segment identity functions of dTRAP240 and dTRAP80, potentially within a common complex. Further, they identify the homeotic Sex combs reduced product, required for the same cell/tissue identities, as a functional partner of these mediator proteins.