141.Khairul Anuar Shariff, Kanji Tsuru, Kunio Ishikawa, Micro-CT evaluation on osteoconductivity of DCPD-coated βTCP granule using experimental rats
141.Khairul Anuar Shariff, Kanji Tsuru, Kunio Ishikawa, Micro-CT evaluation on osteoconductivity of DCPD-coated βTCP granule using experimental rats
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141.Khairul Anuar Shariff、Kanji Tsuru、Kunio Ishikawa，利用实验大鼠对 DCPD 涂层 βTCP 颗粒的骨传导性进行 Micro-CT 评估
DOI：
10.1088/1742-6596/1082/1/012069
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Kunio Ishikawa
中科院分区：
文献类型：
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作者：
Khairul Anuar Shariff;Kanji Tsuru;Kunio Ishikawa
β-磷酸三钙（β-TCP）骨替代材料因其良好的组织反应性和良好的骨传导性而被广泛应用。然而，最近的研究报道，β-TCP的骨传导性可以通过用饱和的酸性磷酸钙溶液处理β-TCP表面来增强。在这项研究中，使用微计算机断层扫描（micro-CT）扫描技术评价处理后的β-TCP颗粒的骨传导性。将β-TCP颗粒分别暴露于饱和酸性磷酸钙溶液中10、30和60 min，获得颗粒样品。然后在大鼠颅骨上制作临界骨缺损，大小为9mm，分别用处理10、30和60 min的β-TCP颗粒和未处理的β-TCP颗粒重建骨缺损。植入2周和4周后，取出带周围骨组织的标本，用微型CT机进行检查。根据显微CT图像的横截面，在所有标本中观察到从缺损边缘向骨缺损区中心的新骨形成。在2周时，与其他标本相比，在10 min和30 min处理的β-TCP颗粒中观察到新骨形成。30 min处理的β-TCP颗粒在植入4周后显示出更快的新骨形成速率。可以得出结论，与其他样本相比，处理30分钟的β-TCP颗粒显示出最高的骨传导性。
β-tricalcium phosphate (β-TCP) bone substitutes have been widely used because of its excellent tissue response and good osteoconductivity. However, recent study reported that the osteoconductivity of β-TCP could be enhanced by treating β-TCP surfaces with saturated acidic calcium phosphate solution. In this study, the osteoconductivity of treated β-TCP granule were evaluated using micro-computed tomography (micro-CT) scan technique. The granule specimens were obtained by exposing β-TCP granules with saturated acidic calcium phosphate solution for 10, 30 and 60 mins, respectively. Then, critical bone defect size with dimension of 9 mm were made at rat calvarial, and the defect was reconstructed with 10, 30 and 60 mins treated β-TCP granules and β-TCP granules without treatment as a control. After 2 and 4 weeks implantation, the specimens with the surrounding bone tissue were taken out and examined using micro-CT machine. Based on the cross section of micro-CT images, new bone formations were observed from the edge of the defect towards the center of the bone defect area in all specimens. At 2 weeks, new bone formations were observed for 10 min and 30 min treated β-TCP granules when compared with other specimens. The 30 min treated β-TCP granules showed faster new bone formation rate after 4 weeks implantation. It can be concluded that 30 min treated β-TCP granules revealed the highest osteoconductivity in comparison to the other specimens.