[Relative expression of SOD and CAT mRNA and activities of SOD and CAT in grafted cucumber leaves under NaCl stress].
[Relative expression of SOD and CAT mRNA and activities of SOD and CAT in grafted cucumber leaves under NaCl stress].
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发表时间：
2008-08
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通讯作者：
Jun-jie Gao;Lin Zhang;Ai-guo Qin;Xian-chang Yu
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作者：
Jun-jie Gao;Lin Zhang;Ai-guo Qin;Xian-chang Yu
以嫁接和自根黄瓜(军绿3号)植株为试材，研究了NaCI胁迫下嫁接和自根黄瓜叶片中铜/锌-超氧化物歧化酶、锰-超氧化物歧化酶和过氧化氢酶基因的相对表达、叶片超氧化物歧化酶、铜/锌-超氧化物歧化酶、锰-超氧化物歧化酶和过氧化氢酶活性、叶片丙二醛含量和叶片电解质渗漏的变化。结果表明，与自根黄瓜相比，盐胁迫下嫁接黄瓜叶片中的铜/锌-超氧化物歧化酶、锰-超氧化物歧化酶和过氧化氢酶的相对表达水平较高，超氧化物歧化酶、铜/锌-超氧化物歧化酶、锰-超氧化物歧化酶和过氧化氢酶的活性也较高，说明嫁接黄瓜叶片中铜/锌-超氧化物歧化酶、锰-超氧化物歧化酶和过氧化氢酶的相对表达水平较高，导致其超氧化物歧化酶、铜/锌-超氧化物歧化酶、锰-超氧化物歧化酶和过氧化氢酶的活性较高。随着盐胁迫时间的延长，嫁接黄瓜和自根黄瓜叶片中铜/锌-超氧化物歧化酶、锰-超氧化物歧化酶和过氧化氢酶的相对表达均呈上升趋势，但铜/锌-超氧化物歧化酶、锰超氧化物歧化酶和过氧化氢酶活性的变化趋势与其mRNA的变化趋势不同，说明超氧化物歧化酶和过氧化氢酶的活性还受其他因素的调节。嫁接黄瓜叶片丙二醛含量和电解质渗漏率均低于自根黄瓜，表明嫁接黄瓜具有较高的清除代谢能力，可减轻氧化损伤，提高其耐盐性。
Taking grafted and own-root cucumber (Junlv No. 3) plants as test materials, the changes of their leaf Cu/Zn-SOD, Mn-SOD and CAT mRNA relative expression, leaf SOD, Cu/Zn-SOD, Mn-SOD and CAT activities, leaf MDA content, and leaf electrolytic leakage under NaCI stress were studied. The results showed that under NaCl stress, grafted cucumber had a higher relative expression of its leaf Cu/Zn-SOD, Mn-SOD and CAT mRNA, and higher activities of its leaf SOD, Cu/Zn-SOD, Mn-SOD and CAT, compared with own-root cucumber, which suggested that the higher relative expression of Cu/Zn-SOD, Mn-SOD and CAT mRNA in grafted cucumber leaves resulted in the higher activities of their SOD, Cu/Zn-SOD, Mn-SOD and CAT. With the NaCl stress prolonged, the relative expression of Cu/Zn-SOD, Mn-SOD and CAT mRNA in both grafted and own-root cucumber leaves had an increasing trend, but the changing trend of Cu/Zn-SOD, MnSOD and CAT activities were not the same as that of mRNA, indicating that the activities of SOD and CAT were also regulated by other factors. The leaf MDA content and electrolytic leakage of grafted cucumber were lower than those of own-root cucumber, suggesting that grafted cucumber had a higher scavenging metabolism, which could decrease oxidative damage and increase its salt-tolerance.