Survival potential of Aeromonas hydrophila in freshwaters and nutrient-poor waters in comparison with other bacteria.
Survival potential of Aeromonas hydrophila in freshwaters and nutrient-poor waters in comparison with other bacteria.
复制标题
与其他细菌相比，嗜水气单胞菌在淡水和营养贫乏水域中的生存潜力。
DOI：
10.1111/j.1365-2672.1996.tb03219.x
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
W. Verstraete
中科院分区：
文献类型：
--
作者：
I. Kersters;G. Huys;H. Van Duffel;M. Vancanneyt;K. Kersters;W. Verstraete
使用活菌计数在水微观世界中研究了基因标记的嗜水气单胞菌菌株的存活情况。研究表明，嗜水气单胞菌 AWWX1 在四种过滤高压灭菌的淡水（地表水和地下水）中的三种以及所有检查的过滤高压灭菌的营养贫乏水（瓶装泉水、Milli-Q 和自来水）中存活至少 10 天，且活菌计数不下降。然而，在未经过滤的水中，活氧数量迅速减少。 1-5天后观察到嗜水菌AWWX1。艾尔的生存。将营养贫乏水域中的嗜水菌 AWWX1 与荧光假单胞菌 P17 和螺菌菌株 NOX 进行比较。生存特征取决于生物体和水。在过滤高压灭菌水中，螺旋菌 NOX 菌株的活菌计数比空气高约 1 个对数单位。嗜水菌 AWWX1 和 Ps。荧光P17。测试菌株 Aer.嗜水菌 AWWX1 和 Ps。与未过滤的水中相比，荧光藻 P17 在过滤高压灭菌水中的存活率分别高出 3 至 20 倍、2 至 4 倍。显然，这些微生物适应低营养环境的任何固有能力都因原生微生物群的存在而被破坏。目前的研究结果表明，Aer。嗜水菌在多种饮用水中的生存能力非常差，这对公共卫生至关重要，并引发了有关其机制的问题。
The survival of a genetically-marked Aeromonas hydrophila strain was studied in water microcosms using viable counts. Aeromonas hydrophila AWWX1 was shown to survive without decline in viable counts for at least 10 d in three of four filtered-autoclaved freshwaters (surface water and groundwater) and in all examined filtered-autoclaved nutrient-poor waters (bottled spring water, Milli-Q and tap water). However, in the unfiltered waters, a rapid decrease in viable counts of Aer. hydrophila AWWX1 was observed after 1-5 d. The survival of Aer. hydrophila AWWX1 in nutrient-poor waters was compared with that of Pseudomonas fluorescens P17 and Spirillum strain NOX. Survival characteristics were organism- and water-dependent. In the filtered-autoclaved waters, viable counts of Spirillum strain NOX were ca 1 log-unit higher than for Aer. hydrophila AWWX1 and Ps. fluorescens P17. The tested strains Aer. hydrophila AWWX1 and Ps. fluorescens P17 survived 3 to 20, respectively 2 to 4 times better in the filtered-autoclaved waters compared to the unfiltered waters. Apparently, any inherent capability of these micro-organisms to adapt to low-nutrient environments was undone by the presence of the autochthonous microbiota. The present findings that Aer. hydrophila survives very poorly in several drinking waters is of utmost importance towards public health and arises questions about the mechanisms involved.