Sensitive to Proton Rhizotoxicity1 Regulates Salt and Drought Tolerance of Arabidopsis thaliana through Transcriptional Regulation of CIPK23
Sensitive to Proton Rhizotoxicity1 Regulates Salt and Drought Tolerance of Arabidopsis thaliana through Transcriptional Regulation of CIPK23
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DOI：
10.1093/pcp/pcz120
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发表时间：
2019-09-01
影响因子：
4.9
通讯作者：
Koyama, Hiroyuki
中科院分区：
文献类型：
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作者：
Sadhukhan, Ayan;Enomoto, Takuo;Koyama, Hiroyuki
对质子根毒性敏感的转录因子1(STOP1)调节多种胁迫耐受性。在本研究中，我们证实了它参与了盐胁迫和耐旱性。STOP1(Stop1)的T-DNA插入突变体(Stop1)的根生长对含盐的MS固体培养基很敏感。盐胁迫下STOP1的转录组分析表明，STOP1调控着几个与耐盐相关的基因，其中包括CIPK23。在所有在stop1中被抑制的基因的纯合子T-DNA插入突变体中，只有cipk23表现出与stop1类似的对盐敏感的根生长表型。CIPK23启动子有一个功能性的STOP1结合位点，这表明CIPK23的强烈抑制导致了Stop1对盐的敏感性。这一可能性得到了CIPK23在Stop1背景下的植物互补的支持，它挽救了盐胁迫下的短根表型。Stop1和cipk23都表现出耐旱表型，并增加了脱落酸调节的气孔关闭，而CIPK23在stop1中的互补逆转了这些特性。我们的发现揭示了由CIPK23介导的STOP1的额外的多效性作用，它调节各种离子转运蛋白，包括那些调节K+稳态的离子转运蛋白，这可能导致耐旱性和其他性状之间的权衡。
The transcription factor sensitive to proton rhizotoxicity 1 (STOP1) regulates multiple stress tolerances. In this study, we confirmed its involvement in NaCl and drought tolerance. The root growth of the T-DNA insertion mutant of STOP1 (stop1) was sensitive to NaCl-containing solidified MS media. Transcriptome analysis of stop1 under NaCl stress revealed that STOP1 regulates several genes related to salt tolerance, including CIPK23. Among all available homozygous T-DNA insertion mutants of the genes suppressed in stop1, only cipk23 showed a NaCl-sensitive root growth phenotype comparable to stop1. The CIPK23 promoter had a functional STOP1-binding site, suggesting a strong CIPK23 suppression led to NaCl sensitivity of stop1. This possibility was supported by in planta complementation of CIPK23 in the stop1 background, which rescued the short root phenotype under NaCl. Both stop1 and cipk23 exhibited a drought tolerant phenotype and increased abscisic acid-regulated stomatal closure, while the complementation of CIPK23 in stop1 reversed these traits. Our findings uncover additional pleiotropic roles of STOP1 mediated by CIPK23, which regulates various ion transporters including those regulating K+-homeostasis, which may induce a trade-off between drought tolerance and other traits.