Long-term surveillance of sulfate-reducing bacteria in highly saline industrial wastewater evaporation ponds.
Long-term surveillance of sulfate-reducing bacteria in highly saline industrial wastewater evaporation ponds.
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DOI：
10.1186/1746-1448-5-2
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发表时间：
2009-02-18
期刊：
影响因子：
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通讯作者：
Brenner, Asher
中科院分区：
文献类型：
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作者：
Ben-Dov, Eitan;Kushmaro, Ariel;Brenner, Asher
在41个月的时间里，研究了高盐工业废水蒸发池中硫酸盐还原菌（SRB）的季节性和季节性动态，特别是极端嗜盐SRB（属于Desulfocella halophila）。对工业废水进行采样，并通过定量实时PCR（qPCR）确定SRB的存在，该PCR使用一组设计用于扩增异化亚硫酸盐还原酶（dsrA）基因的引物。SRB在夏季的丰度较高（10（6）~ 10（8）目标ml（-1）），而在秋季和春季的丰度较低（10（3）~ 10（5）目标ml（-1））。然而，在冬季向蒸发池中加入浓缩的溶解有机物立即导致SRB的增殖，尽管废水温度较低（12-14摄氏度）。这些结果表明，qPCR方法可用于SRB的快速测量，以提供有关SRB在恶劣环境中的丰度的有价值的信息，例如高盐工业废水。低水平的H2S已经维持了五年，这表明在废水蒸发池的人工盐化（约16%w/v的NaCl）之后，可能抑制SRB活性，尽管通过qPCR检测到SRB繁殖。
Abundance and seasonal dynamics of sulfate-reducing bacteria (SRB), in general, and of extreme halophilic SRB (belonging to Desulfocella halophila) in particular, were examined in highly saline industrial wastewater evaporation ponds over a forty one month period. Industrial wastewater was sampled and the presence of SRB was determined by quantitative real-time PCR (qPCR) with a set of primers designed to amplify the dissimilatory sulfite reductase (dsrA) gene. SRB displayed higher abundance during the summer (10(6)-10(8) targets ml(-1)) and lower abundance from the autumn-spring (10(3)-10(5) targets ml(-1)). However, addition of concentrated dissolved organic matter into the evaporation ponds during winter immediately resulted in a proliferation of SRB, despite the lower wastewater temperature (12-14 degrees C). These results indicate that the qPCR approach can be used for rapid measurement of SRB to provide valuable information about the abundance of SRB in harsh environments, such as highly saline industrial wastewaters. Low level of H2S has been maintained over five years, which indicates a possible inhibition of SRB activity, following artificial salination (approximately 16% w/v of NaCl) of wastewater evaporation ponds, despite SRB reproduction being detected by qPCR.