Investigating glutamate receptor-like gene co-expression in Arabidopsis thaliana
Investigating glutamate receptor-like gene co-expression in Arabidopsis thaliana
复制标题
DOI：
10.1111/j.1365-3040.2008.01801.x
复制
发表时间：
2008-06-01
影响因子：
7.3
通讯作者：
Tester, M.
中科院分区：
文献类型：
--
作者：
Roy, S. J.;Gilliham, M.;Tester, M.
越来越多的证据表明，谷氨酸受体（GLRs）在植物发育和逆境适应中起着重要作用。然而，由于我们缺乏对植物中GLR异构体形成的认识，对植物和爪蟾卵母细胞中这些离子通道的研究可能受到限制。我们开发了一种改进的单细胞取样技术来研究GLR共表达，从而研究拟南芥叶片单细胞中潜在的异源异构体的形成。微表达扩增（MEX）使我们能够从单个细胞中扩增基因转录本，从而能够检测多达100个基因转录本的表达。我们平均测量了单个细胞中5到6个不同的atglr的转录本。然而，除了来自同一植物的细胞显示相似的表达谱外，没有检测到一致的共表达模式或细胞类型特异性表达。唯一可识别的特征是在每个被检测的细胞中检测到AtGLR3.7，这一观察结果得到了稳定表达启动子：：uidA融合物的植物的GUS染色模式的支持。此外，我们发现AtGLR3.7在卵母细胞中的表达诱导Ba(2+)-、Ca(2+)-和Na(+)-渗透质膜电导。
There is increasing evidence of the important roles of glutamate receptors (GLRs) in plant development and in adaptation to stresses. However, the studies of these putative ion channels, both in planta and in Xenopus oocytes, may have been limited by our lack of knowledge of possible GLR heteromer formation in plants. We have developed a modification of the single-cell sampling technique to investigate GLR co-expression, and thus potential heteromer formation, in single cells of Arabidopsis thaliana leaves. Micro-EXpression amplification (MEX) has allowed us to amplify gene transcripts from a single cell, enabling expression of up to 100 gene transcripts to be assayed. We measured, on average, the transcripts of five to six different AtGLRs in a single cell. However, no consistent patterns of co-expression or cell-type-specific expression were detected, except that cells sampled from the same plant showed similar expression profiles. The only discernible feature was the detection of AtGLR3.7 in every cell examined, an observation supported by GUS staining patterns in plants stably expressing promoter::uidA fusions. In addition, we found AtGLR3.7 expression in oocytes induces a Ba(2+)-, Ca(2+)- and Na(+)-permeable plasma membrane conductance.