Small peptide regulators of actin-based cell morphogenesis encoded by a polycistronic mRNA
Small peptide regulators of actin-based cell morphogenesis encoded by a polycistronic mRNA
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DOI：
10.1038/ncb1595
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发表时间：
2007-06-01
影响因子：
21.3
通讯作者：
Kageyama, Yuji
中科院分区：
文献类型：
--
作者：
Kondo, Takefumi;Hashimoto, Yoshiko;Kageyama, Yuji
在包括小鼠和人类在内的真核生物中，转录组分析已经确定了含有PolyA的转录本没有长的开放阅读框架(ORF；>；100个氨基酸)1，2。这些转录本被认为最有可能作为非编码RNA发挥功能，但它们的翻译能力和生物活性尚未得到详细描述。在这里，我们报告了抛光米(Pri)，以前被发现是果蝇3，4中非编码RNA的基因，实际上被转录成一个多顺反子mRNA，其中包含进化上保守的短ORF，编码11或32个氨基酸的长肽。在胚胎发育过程中，PRI在所有上皮组织中均有表达。Pri功能的丧失完全消除了顶端角质结构，包括表皮牙和气管带，也导致了有缺陷的气管扩张。我们发现，PrI对于特定的F-肌动蛋白束的形成是必不可少的，F-肌动蛋白束预示着牙节和带的形成。我们提供了PRI自主发挥非细胞作用的证据，并且四个保守的PRI ORF在功能上是多余的。这些结果表明PrI通过调节F-肌动蛋白的组织在上皮的形态发生中起重要作用。
Transcriptome analyses in eukaryotes, including mice and humans, have identified polyA- containing transcripts that lack long open reading frames (ORFs; > 100 amino acids) 1,2. These transcripts are believed most likely to function as non-coding RNAs, but their translational capacities and biological activities have not been characterized in detail. Here, we report that polished rice (pri), which was previously identified as a gene for a non-coding RNA in Drosophila3,4, is in fact transcribed into a polycistronic mRNA that contains evolutionarily conserved short ORFs that encode 11 or 32 amino acid-long peptides. pri was expressed in all epithelial tissues during embryogenesis. The loss of pri function completely eliminated apical cuticular structures, including the epidermal denticles and tracheal taenidia, and also caused defective tracheal-tube expansion. We found that pri is essential for the formation of specific F-actin bundles that prefigures the formation of the denticles and taenidium. We provide evidences that pri acts non-cell autonomously and that four of the conserved pri ORFs are functionally redundant. These results demonstrate that pri has essential roles in epithelial morphogenesis by regulating F-actin organization.