Perfluorocarbon-Encapsulated PLGA-PEG Emulsions as Enhancement Agents for Highly Efficient Reoxygenation to Cell and Organism.
Perfluorocarbon-Encapsulated PLGA-PEG Emulsions as Enhancement Agents for Highly Efficient Reoxygenation to Cell and Organism.
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DOI：
10.1021/acsami.5b04226
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发表时间：
2015-08
影响因子：
9.5
通讯作者：
Yanjie Yao;Minmin Zhang;Tian Liu;Juan Zhou;Yuan Gao;Zhengfeng Wen;Jun Guan;Jun Zhu;
中科院分区：
文献类型：
--
作者：
Yanjie Yao;Minmin Zhang;Tian Liu;Juan Zhou;Yuan Gao;Zhengfeng Wen;Jun Guan;Jun Zhu;
将氧载体全氟化碳（PFC）包裹在聚乳酸-聚乙二醇（PLGA-PEG）中，制备PLGA-PEG/PFC乳剂，对细胞和生物体进行高效复氧。以HCT 116细胞为模型细胞，PLGA-PEG/PFC乳剂复氧后，由于供氧充足及时，HCT 116细胞的存活率显著提高。同时，细胞缺氧复氧处理会沿着发生缺氧复氧损伤，表现为细胞内活性氧（ROS）增加。然而，细胞内活性氧和细胞活力的整合意味着当PLGA-PEG/PFC乳液的浓度低于0.2 mg/mL时，缺氧-复氧损伤的程度对HCT 116细胞是亚致死的。缺氧诱导因子-1 α（hypoxia-inducible factor-1α，HIF-1α）表达水平的变化与复氧过程中细胞活力的变化相似，提示HIF-1α或其下游蛋白可能对细胞活力有重要作用。通过肺给药评价大鼠体内氧供情况，结果表明PLGA-PEG/PFC乳剂能给大鼠供氧，改善大鼠肺通气功能。
Perfluorocarbon (PFC), a kind of oxygen carrier, is encapsulated in PLGA-PEG to prepare a PLGA-PEG/PFC emulsion for highly efficient reoxygenation to cell and organism. HCT 116 cells are used as a model cell, whose viability has a significant enhancement after reoxygenation with PLGA-PEG/PFC emulsion because of the sufficient and timely oxygen supply. Meanwhile, hypoxia-reoxygenation injury will happen along with cell hypoxia-reoxygenation treatment, which is reflected by increasing reactive oxygen species (ROS) in cells. However, the integration of intracellular ROS and cell viability implies that the degree of hypoxia-reoxygenation injury is sublethal to HCT116 cells when the concentration of PLGA-PEG/PFC emulsion is lower than 0.2 mg/mL. Furthermore, the change of the expression level of hypoxia-inducible factor-1α (HIF-1α) is similar to that of cell viability during reoxygenation, which suggests that HIF-1α or its downstream proteins may make a significant contribution to cell viability. In vivo oxygen supply is assessed in rats through pulmonary delivery, which shows that PLGA-PEG/PFC emulsion can supply oxygen to rats and improve rats' lung ventilation.