Detection of HTLV-I proviral sequences in CD30-positive large cell cutaneous T-cell lymphomas.
Detection of HTLV-I proviral sequences in CD30-positive large cell cutaneous T-cell lymphomas.
复制标题
CD30阳性大细胞皮肤T细胞淋巴瘤中HTLV-I前病毒序列的检测。
作者：
I. Anagnostopoulos;M. Hummel;P. Kaudewitz;Hermann Herbst;O. Braun;H. Stein
为了探讨皮肤T细胞淋巴瘤的大细胞型可能与人类T细胞白血病/淋巴瘤病毒I型感染在非流行地区，组织样本6例大细胞皮肤T细胞淋巴瘤和4例小细胞皮肤T细胞淋巴瘤进行了筛选的存在下，集成的前病毒人类T细胞白血病/淋巴瘤病毒I型DNA。Southern印迹杂交和酶促DNA扩增的结合使用揭示了人类T细胞白血病/淋巴瘤病毒I型特异性序列在所有情况下的大细胞皮肤T细胞淋巴瘤和小细胞皮肤T细胞淋巴瘤的情况下没有。这些结果表明，在非流行地区，大细胞皮肤T细胞淋巴瘤病例的显着比例与人类T细胞白血病/淋巴瘤病毒I型。
To investigate the possibility that cutaneous T-cell lymphomas of large cell type may be associated with human T-cell leukemia/lymphoma virus type I infection in nonendemic regions, tissue samples from six cases of large cell cutaneous T-cell lymphoma and four cases of small cell cutaneous T-cell lymphoma were screened for the presence of integrated proviral human T-cell leukemia/lymphoma virus type I DNA. Combined use of Southern blot hybridization and enzymatic DNA amplification revealed human T-cell leukemia/lymphoma virus type I-specific sequences in all cases of large cell cutaneous T-cell lymphoma and in none of the cases of small cell cutaneous T-cell lymphoma. These results suggest that in nonendemic areas, a significant proportion of large cell cutaneous T-cell lymphoma cases are associated with human T-cell leukemia/lymphoma virus type I.