[Analysis of reticulocytes: manual microscopy, flow analyzers or image analyzers? (analytical review)].
[Analysis of reticulocytes: manual microscopy, flow analyzers or image analyzers? (analytical review)].
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发表时间：
2007-10
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通讯作者：
Piatnitskiĭ Am;Medovyĭ Vs;Parpara Aa
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作者：
Piatnitskiĭ Am;Medovyĭ Vs;Parpara Aa
比较了三种测定网织红细胞数量和组成的方法(手动显微镜下的目测分析、流式细胞术、使用计算机显微镜单元的半自动分析)。讨论了每种方法的误差特点和手工分析仍然最常用的原因。文章指出，不能用相关系数的大小来判断程序的精度。为本论文提供了证据，证明只有在使用计算机显微镜装置的图像分析器时才能达到统计精度，因此推荐该方法作为参考。本文介绍了用MEKOS-TS2计算机显微镜装置分析网织红细胞的特点。
Three procedures for determining the quantity and composition of reticulocytes (visual analysis during manual microscopy; flow cytometry, semi-automatic analysis using a computerized microscopy unit) are compared. The errors characteristics for each procedure and the reasons why manual analysis still remains most used are discussed. The paper points out that it is impossible to draw a conclusion on the accuracy of the procedures, by using the values of the correlation coefficient. Evidence is provided for the thesis that the statistical accuracy may be achieved only when the image analyzer of a computerized microscopy unit is employed, therefore this procedure is recommended for use as a reference one. The characteristics of a procedure for analyzing reticulocytes by means of a MEKOS-Ts2 computerized microscopy unit are presented.