Structural features and digestive behavior of fucosylated chondroitin sulfate from sea cucumbers Stichopus japonicus.
Structural features and digestive behavior of fucosylated chondroitin sulfate from sea cucumbers Stichopus japonicus.
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DOI：
10.1021/acs.jafc.9b04996
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发表时间：
2019-08
影响因子：
6.1
通讯作者：
Zhenjun Zhu;Xiu‐ping Dong;Chunhong Yan;Chunqing Ai;Da‐yong Zhou;Jingfeng Yang;Hui Zhang;Xiaoling Liu
中科院分区：
文献类型：
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作者：
Zhenjun Zhu;Xiu‐ping Dong;Chunhong Yan;Chunqing Ai;Da‐yong Zhou;Jingfeng Yang;Hui Zhang;Xiaoling Liu
海参岩藻糖基化硫酸软骨素（FCSSJ）具有多种生物活性，但其结构尚不清楚，消化行为也不清楚。对FCSSJ进行了纯化，并主要基于NMR光谱方法对其详细结构进行了鉴定。其主链结构为→4）-β-D-GlcA-（1→3）-β-D-GalNAc-（1→），其中GalNAc 4S 6S：GalNAc 4S的比例为1.5：1，并发现3种类型的硫酸化岩藻糖基支链连接GlcA的C-3，即Fucp 2S 4S、Fucp 3S 4S和Fucp 4S，比例为2：1.5：1。研究了FCSSJ的体外消化率，FCSSJ的分子量和还原糖含量没有变化，表明FCSSJ在唾液和胃肠道消化中没有被分解。FCSSJ对胰脂肪酶有明显的抑制作用，且呈剂量依赖性，但对α-淀粉酶无明显抑制作用，提示FCSSJ对胰脂肪酶的抑制可能是其降血脂作用的一个途径。这些发现提出了岩藻糖基化硫酸软骨素，并提供了深入了解其在消化系统中的生理作用的机制。
Fucosylated chondroitin sulfate from sea cucumber Stichopus japonicus (FCSSJ) has been demonstrated with various biological activities, however, its precise structure is still controversial, and digestive behavior remains poorly understood. FCSSJ was purified, and its detailed structure was elucidated mainly based on the NMR spectroscopic methods. Its main chain was characterized as →4)-β-D-GlcA-(1→3)-β-D-GalNAc-(1→ with GalNAc4S6S:GalNAc4S in a ratio of 1.5:1, and three types of sulfated fucosyl branches attaching C-3 of GlcA, namely, Fucp2S4S, Fucp3S4S and Fucp4S, were found in a ratio of 2:1.5:1. The digestibility of FCSSJ was investigated in vitro, and the unchanged molecular weight and reducing sugar content indicated that FCSSJ was not broken down under salivary and gastrointestinal digestion. Furthermore, FCSSJ showed a significant inhibitory impact on pancreatic lipase dose-dependently but not on α-amylase, indicating that the inhibition of pancreatic lipase by FCSSJ might be a pathway for its hypolipidemic effect. These findings propose a fucosylated chondroitin sulfate and provide insight into the mechanism of its physiological effects in digestion system.