Endothelial hypoxic stress proteins.
Endothelial hypoxic stress proteins.
复制标题
内皮缺氧应激蛋白。
DOI：
10.1038/ki.1997.57
复制
发表时间：
1997
影响因子：
19.6
通讯作者：
Farber,HW
中科院分区：
文献类型：
--
作者：
Graven,KK;Farber,HW
Fig. 1. BAEC和BPAEC中缺氧诱导蛋白质的时间过程。将细胞维持在21%N2（Cont）中或暴露于0%N2，持续指定的时间。在低氧暴露后，将细胞置于21%O2中，持续两小时恢复期，然后用[35 S]-甲硫氨酸标记一小时。然后将洗涤的细胞溶解在Laemmli样品缓冲液中，煮沸并在10% SDS-PAGE上运行，每个泳道加载相等的计数。MW标准品的位置显示在左侧，诱导蛋白的位置显示在右侧，以kDa计。
Fig. 1. Time course of protein induction by hypoxia in BAEC and BPAEC. Cells were maintained in 21% 2 (Cont) or exposed to 0% 2, for the times indicated. Following hypoxic exposure cells were placed in 21% 02, for a two-hour recovery period then labeled for one hour with [35S1-methionine. Washed cells were then solubilized in Laemmli sample buffer, boiled and run on 10% SDS-PAGE, loading equal counts per lane. The positions of MW standards are shown at the left and positions of induced proteins at the right in kDa.