Immunization with purified protein antigens from Streptococcus mutans against dental caries in rhesus monkeys
Immunization with purified protein antigens from Streptococcus mutans against dental caries in rhesus monkeys
复制标题
使用来自变形链球菌的纯化蛋白抗原对恒河猴进行抗龋齿免疫
DOI：
10.1128/iai.34.2.407-415.1981
复制
发表时间：
1981
影响因子：
3.1
通讯作者：
R. Smith
中科院分区：
文献类型：
--
作者：
T. Lehner;M. Russell;J. Caldwell;R. Smith
蛋白抗原I， I/II， II和III从变形链球菌（血清型c）制备。他们在40只恒河猴身上研究了它们的免疫原性和对龋齿的保护作用，这些恒河猴完全被饲养在含有约15%蔗糖的人类饮食中。与假免疫的猴子相比，抗原I、I/II和抗原II在较小程度上显著减少了蛀牙。这是用1或2剂抗原实现的，第一次是用佐剂（弗氏不完全佐剂或氢氧化铝）。用抗原III免疫的猴子没有减少龋齿。用抗原I或I/II免疫的动物龋齿的减少与用全细胞免疫的猴子相当。对龋齿的保护主要与血清和牙龈沟液免疫球蛋白G抗体有关，这些抗体似乎是针对抗原I决定因素的，但抗原II抗体（尽管不是抗原III抗体）也具有保护作用。
Protein antigens I, I/II, II, and III were prepared from Streptococcus mutans (serotype c). Their immunogenicities and protective effects against dental caries were investigated in 40 rhesus monkeys kept entirely on a human-type diet, containing about 15% sucrose. Antigens I, I/II and, to a lesser extent, antigen II induced significant reductions in dental caries, as compared with sham-immunized monkeys. This was achieved with 1 or 2 doses of antigen, the first of which was administered with adjuvant (Freund incomplete adjuvant or aluminum hydroxide). There was no reduction in caries in monkeys immunized with antigen III. The reduction in caries in the animals immunized with antigens I or I/II was comparable to that in monkeys immunized with whole cells. Protection against caries was associated predominantly with serum and gingival crevicular fluid immunoglobulin G antibodies, which appeared to be directed against the antigen I determinant, but antibodies to antigen II, though not to antigen III, were also protective.