Interleukin-8 modulates feeding by direct action in the central nervous system.
Interleukin-8 modulates feeding by direct action in the central nervous system.
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Interleukin-8 通过直接作用于中枢神经系统来调节进食。
DOI：
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发表时间：
1993
影响因子：
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通讯作者：
J. P. Borkoski
中科院分区：
文献类型：
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作者：
C. Plata;J. P. Borkoski
白细胞介素-8（IL-8）响应于感染、炎症和创伤而释放。在这些病理过程中，IL-8释放的最重要刺激物是IL-1、肿瘤坏死因子和细菌脂多糖（内毒素），这些因子已被证明可抑制摄食。在本研究中，IL-8的参与中枢调节的摄食进行了调查。侧脑室（icv）微量注射重组人IL-8（rhIL-8，1.0-100 ng/只）可抑制大鼠短期（2 h）摄食。rhIL-8的最有效剂量为20 ng，可使2小时摄食量减少25%，夜间摄食量减少23%。侧脑室微量注射抗rhIL-8抗体（200和500 ng）可阻断20 ng rhIL-8对2 h和夜间摄食量的影响。2小时内的行为模式的计算机分析表明，一个特定的减少膳食的大小（33%），而用餐频率和用餐时间没有受到影响后，icv微输注20 ng rhIL-8。icv rhIL-8的这种短期食物摄入抑制伴随着脑脊液-脑和直肠温度的小幅但显著的升高。腹腔注射rhIL-8的剂量相当于那些集中管理没有影响的食物摄入量。结果表明，IL-8直接作用于中枢神经系统以减少摄食。IL-8的这种作用可能有助于经常伴随病理过程的食物摄入抑制。
Interleukin-8 (IL-8) is released in response to infection, inflammation, and trauma. The most important stimuli for IL-8 release during these pathological processes are IL-1, tumor necrosis factor, and bacterial lipopolysaccharide (endotoxin), factors that have been shown to suppress feeding. In the present study, the participation of IL-8 on the central regulation of feeding was investigated. Intracerebroventricular (icv) microinfusion of recombinant human IL-8 (rhIL-8, 1.0-100 ng/rat) suppressed the short-term (2-h) food intake. The most effective dose of rhIL-8, 20 ng, decreased 2-h food intake by 25% and nighttime food intake by 23%. Intracerebroventricular microinfusion of anti-rhIL-8 antibody (200 and 500 ng) blocked the effect of 20 ng rhIL-8 on 2-h and nighttime food intakes. Computerized analysis of behavioral patterns for the 2-h period demonstrated a specific reduction of meal size (by 33%), whereas meal frequency and meal duration were not affected after the icv microinfusion of 20 ng rhIL-8. This short-term food intake suppression by icv rhIL-8 was accompanied by a small, but significant, increase in cerebrospinal fluid-brain and rectal temperatures. Intraperitoneal administration of rhIL-8 in doses equivalent to those administered centrally had no effect on food intake. The results suggest that IL-8 acts directly in the central nervous system to decrease feeding. This effect of IL-8 may contribute to the food intake suppression frequently accompanying pathological processes.