Active efflux common to vincristine and daunorubicin in vincristine-resistant P388 leukemia.
Active efflux common to vincristine and daunorubicin in vincristine-resistant P388 leukemia.
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在长春新碱耐药的 P388 白血病中，长春新碱和柔红霉素常见的主动外排。
DOI：
10.1016/0006-2952(81)90027-7
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发表时间：
1981
影响因子：
5.8
通讯作者：
Yoshio Sakurai
中科院分区：
文献类型：
--
作者：
Makoto Inaba;Reiko Fujikura;Yoshio Sakurai
迄今为止，已经在多种实验肿瘤品系中观察到DNA插入物与长春花生物碱的交叉抗性[l-7]。关于ADR耐药的P388白血病耐药和交叉耐药的化学机制，我们提出不仅蒽环类药物b[8]，放线菌素- d （ACT）和长春花生物碱191的活性也增加。Dan0[1]和Skovsgaard[1]也报道了对柔红霉素耐药的埃利希癌细胞的类似观察结果。由于P388/ADR细胞具有与DNA插入物和vinca生物碱相同的外排系统。最有可能的是，长春新碱（VCR）耐药细胞也能够排除蒽环类药物以及使用类似的运输系统的VCR。在这个问题上，Skovsgaard等已经报道了VCR耐药的埃利希癌细胞具有与VCR和柔红素（DAU）相同的能量依赖性药物挤出。在这里，我们提出的证据表明P388NCR细胞也被赋予了增强的向外运输这些不同类型的dkua的原因。材料和方法。如先前报道，通过体内程序建立了一种葡萄~耐药P388兄弟链（P388NCR）。简而言之，抗性亚系细胞通过每天（第1 -9天）用0.25 mgikg的VCR处理，仅在第一代易感。体外灵敏度测定采用前文中介绍的原代悬浮培养技术
To date, cross-resistance between DNA intercalaters and vinca alkaloids has been observed with a variety of experimental tumor lines [l-7]. With regard to b~ ochemicai mechanism of resistance and cross-resistance in ad~ amy~ n (ADR)-resistant P388 Ieukemia, we proposed an increased active effiux of not only anthracyclines [8] but actinomycin-D (ACT) and vinca alkaloids 191. Dan0 [lo] and Skovsgaard [ll] also reported the similar observation with daunorubicin-resistant Ehrlich carcinoma cells. Since P388/ADR cells possess an efflux system common to DNA intercalaters and vinca alkaloids. it is most likelv that vincristine (VCR)-resistant cells are also able ti exclude anthracyclines as well as VCR using the similar transport system. On this subject, Skovsgaard [12] already reported that VCR-resistant Ehrlich carcinoma cells possess an energy-dependent drug extrusion common to VCR and daunorubin (DAU). Here we present evidence that P388NCR cells are also endowed with enhanced cauacitv for outward transport of those different classes of dkua.’material and methods. A vinc~ stine-resistant P388 sibline (P388NCR) was established by in vivo procedure, as reported previously 1131. In brief, the resistant subline cells were selected by daily treatment (day l-9) with 0.25 mgikg of VCR onlv on the first transolant generation. In oitro sensitivity was determined by th’e primary suspension culture technique, which was described in the previous daper