Effect of Low Power Laser Irradiation on the Ability of Cell Growth and Myogenic Differentiation of Myoblasts Cultured In Vitro
Effect of Low Power Laser Irradiation on the Ability of Cell Growth and Myogenic Differentiation of Myoblasts Cultured In Vitro
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DOI：
10.1155/2014/290765
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发表时间：
2014-07
影响因子：
3.2
通讯作者：
Cuiping Zhang;Shaohua Li;Xiao-Ying Wang;Peng Chen;Chang-Zhen Wang;Xiaobing Fu;H. Kang;B. Shen;Jie Liang
中科院分区：
文献类型：
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作者：
Cuiping Zhang;Shaohua Li;Xiao-Ying Wang;Peng Chen;Chang-Zhen Wang;Xiaobing Fu;H. Kang;B. Shen;Jie Liang
作为一种治疗方式，低功率激光照射（LPLI）已在临床上用于治疗骨骼肌损伤和其他肌病疾病，但这种治疗的细胞和分子机制仍不清楚。成肌细胞是静止于成熟骨骼肌纤维中的一类肌源性干细胞，被认为是骨骼肌再生过程中的源细胞。本研究旨在探讨LPLI对体外培养的成肌细胞增殖和成肌分化的影响，并寻找LPLI诱导肌肉再生的主要候选细胞。本研究采用氦氖激光照射原代大鼠成肌细胞。通过形态学观察和分子生物学方法监测细胞增殖、分化和细胞对LPLI的反应。结果发现，一定剂量的LPLI可提高成肌细胞的生长潜能，并诱导更多的细胞进入S期，表现为高水平的BrdU掺入，同时在一定程度上抑制成肌细胞的体外分化，并降低成肌调控基因的表达。这些结果为LPLI在骨骼肌再生中的临床应用提供了实验依据。
As a therapeutic modality, low power laser irradiation (LPLI) has been used clinically in the treatment of skeletal muscle injuries and other myopathic conditions, but the cellular and molecular mechanisms attributed to this therapy were still unclear. Myoblasts are a type of myogenic stem cells quiescence in mature skeletal muscle fibers and are considered as the source cells during the regenerating process. The purpose of this paper was to investigate the effects of LPLI on the proliferation and myogenic differentiation of the cultured myoblasts and to find out the major candidates responsible for LPLI-induced muscle regeneration in vivo. In this study, primary rat myoblasts were exposed to helium-neon (He-Ne) laser. Cell proliferation, differentiation, and the cellular responses to LPLI were monitored by using morphological observation and molecular biological methods. It was found that LPLI at a certain fluence could increase the cell growth potential for myoblasts and further induce more cells entering into S phase of the mitotic cycle as indicated by high levels of bromodeoxyuridine (BrdU) incorporation, while at the same time inhibiting their in vitro differentiation and decreasing the expression of myogenic regulatory genes to a certain extent. Taken together, these results provide experimental evidence for the clinical applications of LPLI in regenerating skeletal muscle.