Nonradioactive northern blotting for the determination of acetylcholinesterase mRNA. Comparison to the radioactive technique
Nonradioactive northern blotting for the determination of acetylcholinesterase mRNA. Comparison to the radioactive technique
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用于测定乙酰胆碱酯酶 mRNA 的非放射性 Northern 印迹。
DOI：
10.1007/s004240000094
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Z. Grubic
中科院分区：
文献类型：
--
作者：
K. Z. Kreft;S. Kreft;R. Komel;Z. Grubic
一个敏感的非放射性北方印迹检测哺乳动物组织中的乙酰胆碱酯酶mRNA的描述和比较，其放射性版本。如果地高辛标记的RNA探针用于杂交，CDP-Star™（一种荧光碱性磷酸酶底物）用于检测，则获得最佳结果。所描述的非放射性技术的乙酰胆碱酯酶mRNA的测定是敏感的放射性的，但不需要对辐射的保护，是耗时较少。由于标记探针的稳定性更高，从实验计划的角度来看，非放射性技术也更方便。
A sensitive nonradioactive northern blotting for the detection of acetylcholinesterase mRNA in mammalian tissues is described and compared to its radioactive version. Best results were obtained if digoxigenin labeled RNA probe was used for hybridization and CDP-Star™, a chemiluminescent alkaline phosphatase substrate, for detection. The described nonradioactive technique for acetylcholinesterase mRNA determination is as sensitive as the radioactive one, but requires no protection against radiation and is less time consuming. Because of higher stability of the labeled probe, nonradioactive technique is also more convenient from the standpoint of experimental planning.