Ageing changes in the tensile properties of tendons: Influence of collagen fibril volume fraction
Ageing changes in the tensile properties of tendons: Influence of collagen fibril volume fraction
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DOI：
10.1115/1.2898732
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发表时间：
2008-04-01
影响因子：
1.7
通讯作者：
Wess, T. J.
中科院分区：
文献类型：
--
作者：
Goh, K. L.;Holmes, D. F.;Wess, T. J.
结缔组织是生物复合材料，由嵌入（并增强）细胞外基质 (ECM) 内富含水合蛋白聚糖 (PG) 凝胶的胶原纤维组成。与年龄相关的组织机械特性的变化通常与 ECM 结构（即原纤维直径）的变化有关。然而，将原纤维直径与机械性能关联起来的定量尝试却没有得出结论性的证据。在这里，我们描述了一种基于纤维复合材料混合物规则的新方法，用于评估肌腱拉伸强度 (sigma) 和刚度 (E) 与年龄相关的变化对胶原纤维横截面积分数 (rho) 的依赖性。与原纤维体积分数有关。来自年龄组1.6-35.3月龄的C57BL6小鼠的尾腱被拉伸至无法确定西格玛和E。使用透射电子显微镜对作为年龄函数的rho进行平行测量。使用在肌腱中增强 PG 凝胶的原纤维的数学模型（混合物规则）来研究 rho 对 sigma 和 E 的老化变化的影响。sigma、E 和 rho 的 s 从 1.6 个月到 4.0 个月迅速增加（P 值大小 < 0.05），然后从 4.0 个月到 29.0 个月达到恒定值（与年龄无关）（P 值 > 0.05）； E 和 rho（P 值 > 0.05）的这种趋势从 29.0 个月持续到 35.3 个月，但 sigma 则不然，它逐渐下降（P 值 < 0.05）。线性回归分析显示，与年龄相关的 sigma 和 E 变化与 p 呈正相关（P 值 < 0.05）。胶原原纤维横截面积分数 rho 是 C57BL6 小鼠尾腱中 sigma 和 E 衰老变化的重要预测因子。
Connective tissues are biological composites comprising of collagen fibrils embedded in (and reinforcing) the hydrated proteoglycan-rich (PG) gel within the extracellular matrices (ECMs). Age-related changes to the mechanical properties of tissues are often associated with changes to the structure of the ECM, namely, fibril diameter. However, quantitative attempts to correlate fibril diameter to mechanical properties have yielded inconclusive evidence. Here, we described a novel approach that was based on the rule of mixtures for fiber composites to evaluate the dependence of age-related changes in tendon tensile strength (sigma) and stiffness (E) on the collagen fibril cross-sectional area fraction (rho). which is related to the fibril volume fraction. Tail tendons from C57BL6 mice from age groups.1.6-35.3 months old were stretched to failure to determine sigma and E. Parallel measurements of rho as a function of age were made using transmission electron microscopy. Mathematical models (rule of mixtures) of fibrils reinforcing a PG gel in tendons were used to investigate the influence of rho on ageing changes in sigma and E. The s of sigma, E, and rho increased rapidly from 1.6 months to 4.0 months (P-values magnitude < 0.05) before reaching a constant (age independent) from 4.0 months to 29.0 months (P-values > 0.05); this trend continued for E and rho (P-values > 0.05) from 29.0 months to 35.3 months, but not for sigma, which decreased gradually (P-values < 0.05). Linear regression analysis revealed that age-related changes in sigma and E correlated positively to p (P-values < 0.05). Collagen fibril cross-sectional area fraction rho is a significant predictor of ageing changes in sigma and E in the tail tendons of C57BL6 mice.