Spermatocyte-specific gene excision by targeted expression of Cre recombinase
Spermatocyte-specific gene excision by targeted expression of Cre recombinase
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DOI：
10.1006/bbrc.2000.2762
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发表时间：
2000-05-27
影响因子：
3.1
通讯作者：
Nakanishi, Y
中科院分区：
文献类型：
--
作者：
Ando, H;Haruna, Y;Nakanishi, Y
产生了携带Cre重组酶编码序列的转基因小鼠，Cre重组酶的表达由精母细胞特异性Pgk-8启动子驱动。这些小鼠与报告基因转基因系杂交，该转基因系根据loxP介导的DNA重组的发生产生β-半乳糖苷酶。当通过PCR和Southern印迹分析后代的DNA时，重组后出现的信号仅在睾丸中可检测到。组织化学分析表明，β-半乳糖苷酶存在于精母细胞和生精细胞在分化后期阶段。然而，该蛋白在所有精母细胞中的分布并不均匀。对下一代的基因组DNA分析表明，约70%的生精细胞发生了重组。从这些结果中，我们得出结论，这个转基因株系具有Pgk-2驱动的表达的Cre重组酶应该是有用的，用于确定生精基因的功能在精母细胞阶段或之后。(C)北京大学出版社.
Transgenic mice carrying the coding sequence of the Cre recombinase, whose expression was driven by the spermatocyte-specific Pgk-8 promoter, were generated. These mice were crossed with a reporter transgenic line, which produces beta-galactosidase depending on the occurrence of loxP-mediated DNA recombination. When DNA of the offspring was analyzed by PCR and Southern blotting, signals that appear after the recombination were detectable only in the testis. Histochemical analyses revealed that beta-galactosidase was present in spermatocytes and spermatogenic cells at later differentiation stages. However, the distribution of the protein was not uniform in all spermatocytes. Analyses of genomic DNA of the next generation indicated that recombination took place in about 70% of spermatogenic cells. From these results, we concluded that this transgenic line possessing Pgk-2-driven expression of the Cre recombinase should be useful for identifying spermatogenic genes that function at or after the spermatocyte stage. (C) 2000 Academic Press.