A cellulose synthase-like protein is required for osmotic stress tolerance in Arabidopsis.
A cellulose synthase-like protein is required for osmotic stress tolerance in Arabidopsis.
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DOI：
10.1111/j.1365-313x.2010.04227.x
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发表时间：
2010-07-01
期刊：
影响因子：
--
通讯作者：
Zhu JK
中科院分区：
文献类型：
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作者：
Zhu J;Lee BH;Dellinger M;Cui X;Zhang C;Wu S;Nothnagel EA;Zhu JK
土壤盐分和干旱胁迫造成的渗透胁迫显着影响植物的生长和发育，但渗透胁迫感知和耐受机制尚不清楚。使用根部弯曲测定的正向遗传筛选先前已鉴定出拟南芥盐过度敏感 (sos) 突变体，该突变体分为五个基因座：SOS1 至 SOS5。这些基因座是在盐胁迫下调节离子稳态或细胞扩张所必需的，但在植物对土壤盐分或干旱的渗透胁迫成分的耐受性中不起主要作用。在这里，我们报告了一个额外的 sos 突变体，sos6-1，它定义了渗透胁迫耐受性所必需的基因座。 sos6-1 植物对培养基中的甘露醇或聚乙二醇或土壤中的水分缺乏造成的盐胁迫和渗透胁迫高度敏感。 SOS6 编码纤维素合酶样蛋白 AtCSLD5。只能检测到细胞壁化学成分的微小差异，但我们发现 sos6-1 突变植物在渗透胁迫下积累高水平的活性氧（ROS），并且对氧化应激试剂甲基紫精高度敏感。结果表明，SOS6/AtCSLD5不是正常植物生长和发育所必需的，但在渗透胁迫耐受性中具有关键作用，并且该功能可能涉及其在胁迫下对ROS的调节。
Osmotic stress imposed by soil salinity and drought stress significantly affects plant growth and development, but osmotic stress sensing and tolerance mechanisms are not well understood. Forward genetic screens using a root-bending assay have previously identified salt overly sensitive (sos) mutants of Arabidopsis that fall into five loci, SOS1 to SOS5. These loci are required for the regulation of ion homeostasis or cell expansion under salt stress, but do not play a major role in plant tolerance to the osmotic stress component of soil salinity or drought. Here we report an additional sos mutant, sos6-1, which defines a locus essential for osmotic stress tolerance. sos6-1 plants are hypersensitive to salt stress and osmotic stress imposed by mannitol or polyethylene glycol in culture media or by water deficit in the soil. SOS6 encodes a cellulose synthase-like protein, AtCSLD5. Only modest differences in cell wall chemical composition could be detected, but we found that sos6-1 mutant plants accumulate high levels of reactive oxygen species (ROS) under osmotic stress and are hypersensitive to the oxidative stress reagent methyl viologen. The results suggest that SOS6/AtCSLD5 is not required for normal plant growth and development but has a critical role in osmotic stress tolerance and this function likely involves its regulation of ROS under stress.