Genome Analyses of Icelandic Strains of Sulfolobus islandicus, Model Organisms for Genetic and Virus-Host Interaction Studies
Genome Analyses of Icelandic Strains of Sulfolobus islandicus, Model Organisms for Genetic and Virus-Host Interaction Studies
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冰岛硫化叶菌菌株的基因组分析，用于遗传和病毒-宿主相互作用研究的模型生物
DOI：
10.1128/jb.01487-10
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发表时间：
2011-04-01
影响因子：
3.2
通讯作者：
Garrett, Roger A.
中科院分区：
文献类型：
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作者：
Guo, Li;Brugger, Kim;Garrett, Roger A.
对从冰岛solfataras中获得的2株岛硫虫（Sulfolobus islandicus）进行基因组测序和分析。菌株REY15A是一个多功能遗传工具箱的宿主。它的基因组最小，遗传稳定，易于生长和操作。菌株HVE10/4对特殊的古细菌病毒和共轭质粒具有广泛的宿主范围，并被选中用于研究它们的生命周期和宿主相互作用。菌株REY15A和HVE10/4的基因组分别为2.5 Mb和2.7 Mb，每个基因组携带0.5 ~ 0.7 Mb的可变区，其中基因含量和基因顺序存在较大差异。这些包括参与特定代谢途径的基因簇，VapBC抗毒素-毒素基因对的多个拷贝，以及菌株HVE10/4中富含糖基转移酶基因的50 kb区域。可变区还包含大部分插入序列（IS）元件和高比例的孤儿orfB元件和SMN1微型倒置重复转座元件（MITEs），以及基于簇状规则间隔短回文重复（CRISPR）的免疫系统，这些免疫系统在两种菌株中都是复杂多样的，与它们在细胞内和细胞间都被动员一致。相比之下，其余的基因组在蛋白质和RNA基因共合体上高度保守，与其他S. islandicus和Sulfolobus solfataricus菌株非常相似，并且它们只表现出少数遗传元件的少量残余，主要是共轭质粒，它们整合了一些缺乏内含子的tRNA基因。这为内含子的存在提供了一个可能的理论基础。
The genomes of two Sulfolobus islandicus strains obtained from Icelandic solfataras were sequenced and analyzed. Strain REY15A is a host for a versatile genetic toolbox. It exhibits a genome of minimal size, is stable genetically, and is easy to grow and manipulate. Strain HVE10/4 shows a broad host range for exceptional crenarchaeal viruses and conjugative plasmids and was selected for studying their life cycles and host interactions. The genomes of strains REY15A and HVE10/4 are 2.5 and 2.7 Mb, respectively, and each genome carries a variable region of 0.5 to 0.7 Mb where major differences in gene content and gene order occur. These include gene clusters involved in specific metabolic pathways, multiple copies of VapBC antitoxin-toxin gene pairs, and in strain HVE10/4, a 50-kb region rich in glycosyl transferase genes. The variable region also contains most of the insertion sequence (IS) elements and high proportions of the orphan orfB elements and SMN1 miniature inverted-repeat transposable elements (MITEs), as well as the clustered regular interspaced short palindromic repeat (CRISPR)-based immune systems, which are complex and diverse in both strains, consistent with them having been mobilized both intra- and intercellularly. In contrast, the remainder of the genomes are highly conserved in their protein and RNA gene syntenies, closely resembling those of other S. islandicus and Sulfolobus solfataricus strains, and they exhibit only minor remnants of a few genetic elements, mainly conjugative plasmids, which have integrated at a few tRNA genes lacking introns. This provides a possible rationale for the presence of the introns.