Inhibition of juvenile hormone biosynthesis in mosquitoes: effect of allatostatic head factors, PISCF- and YXFGL-amide-allatostatins
Inhibition of juvenile hormone biosynthesis in mosquitoes: effect of allatostatic head factors, PISCF- and YXFGL-amide-allatostatins
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DOI：
10.1016/j.regpep.2003.12.004
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发表时间：
2004-05-15
影响因子：
--
通讯作者：
Noriega, FG
中科院分区：
文献类型：
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作者：
Li, YP;Hernandez-Martinez, S;Noriega, FG
研究了头部因子和allatostatin（AS）对雌成蚊保幼激素（JH）合成的调节作用。埃及伊蚊（Aedes aegypti）咽侧体（CA）在体外的生物合成活性受到头部因子的抑制。将CA与大脑断开导致JH生物合成速率的显著增加。抑制是不依赖于完整的神经连接，CA与大脑或脑提取物共孵育导致JH生物合成的显着减少。脑提取物的这种抑制作用是可逆的和热稳定的;提取物在蛋白酶K消化后失去了抑制活性，表明肽结构。在第一次尝试阐明这种抑制因子的性质，我们测试了在我们的CA在体外系统的两个家庭的allatostatin已经描述了蚊子的成员的影响。冈比亚按蚊PISCF-allatostatin（Manduca PISCF-allatostatin的同源物）显著抑制JH合成，而Ae.埃及YXFGL-酰胺-allatostatin（蟑螂YXFGL-酰胺-allatostatin的同系物）不影响JH合成。这些结果代表了在鳞翅目昆虫之外PISCF-allatostatins的异育抑制作用的首次描述。(C)2004 Elsevier B. V.保留所有权利。
We investigated the role of head factors and allatostatins (ASs) on the regulation of juvenile hormone (JH) synthesis in female adult mosquito. The biosynthetic activity of the Aedes aegypti corpora allata (CA) in vitro was inhibited by factors present in the head. Disconnecting the CA from the brain resulted in a significant increase in the rate of JH biosynthesis. Inhibition was not dependent on intact nervous connections; co-incubation of CA with brains or brain extracts resulted in a significant decrease of JH biosynthesis. This inhibitory effect of brain extracts was reversible and heat stable; extracts lost the inhibitory activity after proteinase K digestion suggesting a peptidic structure. In a first attempt to elucidate the nature of this inhibitory factor, we tested in our CA in vitro system the effect of members of two families of allatostatins already described in mosquitoes. Anopheles gambiae PISCF-allatostatin (homolog to Manduca PISCF-allatostatin) significantly inhibited JH synthesis, while Ae. aegypti YXFGL-amide-allatostatins (homologs to cockroach YXFGL-amide-allatostatins) did not affect JH synthesis. These results represent the first description of an allatostatic effect of PISCF-allatostatins outside the Lepidoptera. (C) 2004 Elsevier B.V. All rights reserved.