Caffeine improves adult mice performance in the object recognition task and increases BDNF and TrkB independent on phospho-CREB immunocontent in the hippocampus
Caffeine improves adult mice performance in the object recognition task and increases BDNF and TrkB independent on phospho-CREB immunocontent in the hippocampus
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DOI：
10.1016/j.neuint.2008.06.006
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发表时间：
2008-09-01
影响因子：
4.2
通讯作者：
Porciuncula, Lisiane O.
中科院分区：
文献类型：
--
作者：
Costa, Marcelo S.;Botton, Paulo H.;Porciuncula, Lisiane O.
咖啡因是世界上消耗最多的精神兴奋剂之一，通常对认知产生积极影响。本研究在不同的实验间隔时间内，考察了咖啡因对小鼠物体再认能力的影响。此外，还分析了咖啡因对脑源性神经营养因子（BDNF）及其受体TrkB免疫含量的影响，试图建立与记忆和学习过程密切相关的神经营养因子BDNF之间的联系。CH小鼠在连续4天期间用盐水（0.9g %，i. p.）或咖啡因（10 mg/kg，i. p.，相当于2-3杯咖啡的剂量）。在物体识别任务分析前24小时中断咖啡因治疗。在训练15分钟后进行的测试中，咖啡因处理的小鼠更有效地识别熟悉和新物体。在训练后90分钟和24小时进行的测试中，与对照组相比，咖啡因没有改变在熟悉物体上花费的时间，但增加了物体识别指数。对咖啡因处理的小鼠海马的蛋白质印迹分析显示，与盐水对照组相比，BDNF和TrkB免疫含量增加。磷酸化CREB免疫含量没有改变与咖啡因治疗。我们的研究结果表明，急性治疗与咖啡因改善再认记忆，这种效果可能与增加BDNF和TrkB在海马中的免疫含量。(C)2008爱思唯尔有限公司版权所有。
Caffeine is one of the most psychostimulants consumed all over the world that usually presents positive effects on cognition. In this study, effects of caffeine on mice performance in the object recognition task were tested in different intertrial intervals. In addition, it was analyzed the effects of caffeine on brain derived neurotrophic factor (BDNF) and its receptor, TrkB, immunocontent to try to establish a connection between the behavioral finding and BDNF, one of the neurotrophins strictly involved in memory and learning process. CH mice were treated during 4 consecutive days with saline (0.9 g%, i.p.) or caffeine (10 mg/kg, i.p., equivalent dose corresponding to 2-3 cups of coffee). Caffeine treatment was interrupted 24 h before the object recognition task analysis. In the test session performed 15 min after training session, caffeine-treated mice recognized more efficiently both the familiar and the novel object. In the test session performed 90 min and 24 h after training session, caffeine did not change the time spent in the familiar object but increased the object recognition index, when compared to control group. Western blotting analysis of hippocampus from caffeine-treated mice revealed an increase in BDNF and TrkB immunocontent, compared to their saline-matched controls. Phospho-CREB immunocontent did not change with caffeine treatment. Our results suggest that acute treatment with caffeine improves recognition memory, and this effect may be related to an increase of the BDNF and TrkB immurrocontent in the hippocampus. (C) 2008 Elsevier Ltd. All rights reserved.